Depletion of abundant proteins from non-human primate serum for biomarker studies

Proteomics
Willard M FreemanKent E Vrana

Abstract

Non-human primates are an important biomedical research model organism and offer great promise for serum biomarker proteomic studies. However, potential obstacles to these studies include affinity serum depletion methods based on human antigens, depletion methods altering quantitation, and incomplete non-human primate genome sequences for protein identification. In the present study, high-abundance protein removal from monkey serum using a human multiple affinity removal system (MARS) was shown to be specific and did not alter quantitation. Depleted serum also demonstrated greater sensitivity for previously masked, lower-abundance proteins.

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Citations

Nov 15, 2007·Stem Cells and Development·Miguel BartheleryKent E Vrana
Mar 5, 2008·Pediatric Research·Melinda E LullAkif Undar
Jan 22, 2011·Journal of Cancer Research and Clinical Oncology·Masami KadowakiMasaru Miyazaki
Jun 6, 2013·Science China. Life Sciences·Yang ZhangPengyuan Yang
Feb 18, 2010·Proteomics. Clinical Applications·Melinda E LullWillard M Freeman
Apr 4, 2008·Proteomics·Miguel BarthéléryKent E Vrana
Jun 28, 2011·Electrophoresis·Chiara FaniaCecilia Gelfi
Feb 10, 2011·The International Journal of Neuropsychopharmacology·Willard M FreemanKent E Vrana
May 15, 2015·PloS One·Jin-Gyun LeeSunil Hwang
Apr 4, 2006·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Willard M FreemanKent E Vrana
Oct 22, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Todd M UmsteadDavid S Phelps
May 5, 2007·Analytical Chemistry·Jeffrey C SmithDaniel Figeys

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