PMID: 8967517May 1, 1996Paper

Depletion of alveolar macrophages decreases neutrophil chemotaxis to Pseudomonas airspace infections

The American Journal of Physiology
S HashimotoJ P Wiener-Kronish

Abstract

The mechanism for neutrophil (PMN) influx into infected airspaces of the lung is not known. To determine whether alveolar macrophage products are important in the initiation of chemotaxis, we depleted rats of alveolar macrophages by aerosolizing negatively charged oligolamellar liposomes complexed to clodronate disodium. Ninety-five percent of the alveolar macrophages were depleted, and lung injury and inflammation were minimized with this depletion technique. Rats depleted of alveolar macrophages were then anesthetized, and either 5 x 10(6) colony-forming units (CFU) or 5 x 10(7) CFU of Pseudomonas aeruginosa were instilled into the airspaces of these animals. When recombinant macrophage inflammatory protein MIP-2 was intratracheally instilled into rats depleted of alveolar macrophages, PMN were recruited to their airspaces. Nonetheless, PMN numbers were decreased in the lavage fluids when moderate or large inoculums of bacteria were instilled into depleted rats, although the PMN response appeared dose dependent. Levels of bioactive tumor necrosis factor-alpha and immunoreactive proteins CINC/gro (cytokine-induced PMN chemoattractant) in the lavage fluids obtained from infected rats depleted of alveolar macrophages were signif...Continue Reading

Citations

Apr 24, 2002·American Journal of Respiratory Cell and Molecular Biology·M Audrey KoayJohn W Christman
Oct 31, 2002·American Journal of Respiratory and Critical Care Medicine·Sylvia KnappTom van der Poll
Nov 18, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lee J QuintonGregory J Bagby
May 12, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ann M LeeDavid B Jacoby
May 1, 2007·Intensive Care Medicine·Fabien G EyalJames C Parker
Oct 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·J FanA B Malik
Aug 16, 2005·American Journal of Respiratory Cell and Molecular Biology·Adeline M HajjarShawn J Skerrett
Aug 1, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·James A FrankMichael A Matthay
Feb 5, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Becky J ProskocilAllison D Fryer
May 11, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ulrich A MausJürgen Lohmeyer
Jun 17, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·R A BemG Matute-Bello
Jun 22, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazutoshi HamanakaJames C Parker
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·N IsowaM Liu
Jun 28, 2003·American Journal of Respiratory Cell and Molecular Biology·Jeff N VanderbiltLeland G Dobbs
Jun 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert PaineSteven E Wilcoxen
Jul 16, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gustavo Matute-BelloThomas R Martin
Nov 26, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kiyoyasu KurahashiJeanine P Wiener-Kronish
May 17, 2018·Physiological Reviews·Lee J QuintonJoseph P Mizgerd
May 1, 2021·International Journal of Molecular Sciences·Hiromasa TannoKazuyoshi Kawakami

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