Depletion of CD4 T lymphocytes in human lymphoid tissue infected ex vivo with doxycycline-dependent HIV-1

Virology
Yana KiselyevaL Margolis

Abstract

We investigated whether CD4+ T cells that do not produce HIV-1 are killed in HIV-infected human lymphoid tissue. Tissue blocks were inoculated with high amount of doxycycline-dependent HIV-rtTA. Doxycycline triggered productive infection and loss of CD4+ T cells in these tissues, whereas without doxycycline, neither productive infection nor CD4+ T cell depletion was detected in spite of the massive presence of virions in the tissue and of viral DNA in the cells. Thus, HIV-1 alone is sufficient to deplete productively infected CD4+ T cells but is not sufficient to cause the death of uninfected or latently infected CD4+ T cells.

References

Jul 10, 1992·Science·L MeyaardF Miedema
Oct 1, 1992·The Journal of Experimental Medicine·N K BandaT H Finkel
Nov 1, 1991·AIDS Research and Human Retroviruses·M A Gama SosaR M Ruprecht
May 28, 1993·Science·M L Gougeon, L Montagnier
Jan 1, 1997·Current Opinion in Hematology·C R Casella, T H Finkel
Jan 1, 1997·Springer Seminars in Immunopathology·G PantaleoA S Fauci
May 16, 1998·The Journal of Experimental Medicine·R T GandhiD Baltimore
Nov 9, 2000·Genes & Development·R W Doms, D Trono
Apr 26, 2001·Apoptosis : an International Journal on Programmed Cell Death·Y Yang, J D Ashwell
Apr 27, 2001·Trends in Immunology·U Mahlknecht, G Herbein
Apr 3, 2002·Nature Medicine·Zvi GrossmanWilliam E Paul
Apr 12, 2003·AIDS Research and Human Retroviruses·Jean-Charles GrivelLeonid B Margolis
Aug 7, 2003·Expert Review of Vaccines·Atze T DasBen Berkhout
Feb 6, 2004·The Journal of Biological Chemistry·Atze T DasBen Berkhout

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Citations

Jun 29, 2010·HIV Therapy·Atze T DasBen Berkhout
Jun 1, 2012·The Journal of General Virology·Nicolas LegrandMireille Centlivre
May 20, 2015·Virus Research·Atze T Das, Ben Berkhout
May 16, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ellen M WesterhoutBen Berkhout

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