Depletion of cellular pre-replication complex factors results in increased human cytomegalovirus DNA replication.

PloS One
Tamara Evans BraunJohn Sinclair

Abstract

Although HCMV encodes many genes required for the replication of its DNA genome, no HCMV-encoded orthologue of the origin binding protein, which has been identified in other herpesviruses, has been identified. This has led to speculation that HCMV may use other viral proteins or possibly cellular factors for the initiation of DNA synthesis. It is also unclear whether cellular replication factors are required for efficient replication of viral DNA during or after viral replication origin recognition. Consequently, we have asked whether cellular pre-replication (pre-RC) factors that are either initially associated with cellular origin of replication (e.g. ORC2), those which recruit other replication factors (e.g. Cdt1 or Cdc6) or those which are subsequently recruited (e.g. MCMs) play any role in the HCMV DNA replication. We show that whilst RNAi-mediated knock-down of these factors in the cell affects cellular DNA replication, as predicted, it results in concomitant increases in viral DNA replication. These data show that cellular factors which initiate cellular DNA synthesis are not required for the initiation of replication of viral DNA and suggest that inhibition of cellular DNA synthesis, in itself, fosters conditions which ...Continue Reading

References

Jul 1, 1991·The Journal of General Virology·P F ErtlK L Powell
Aug 15, 1998·The Journal of Biological Chemistry·W A BresnahanE A Thompson
Aug 10, 2000·Journal of Virology·J P CastilloT F Kowalik
Dec 23, 2000·Science·J A WohlschlegelA Dutta
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·B ChaudhuriJ L Yates
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoon-Jae Song, Mark F Stinski
Nov 5, 2002·Journal of Virology·Emanuela NorisSanto Landolfo
Jan 14, 2003·EMBO Reports·Lüder WiebuschChristian Hagemeier
Mar 13, 2003·European Journal of Biochemistry·Yukio IshimiSi-Young Song
Apr 7, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Supriya G PrasanthBruce Stillman
Oct 18, 2005·The Journal of General Virology·Cora GriffinPeter Tomasec
Oct 18, 2005·Nature Structural & Molecular Biology·Christian SpeckBruce Stillman
Jan 3, 2006·Molecular Cell·John C W RandellStephen P Bell
Jan 28, 2006·EMBO Reports·Eric LauWei Jiang
Feb 23, 2007·Cell Cycle·Lance F Da-Silva, Bernard P Duncker

❮ Previous
Next ❯

Citations

Mar 17, 2015·Medical Microbiology and Immunology·Emma Poole, John Sinclair

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
FCS
transfection
X-ray
fluorescence microscopy
flow cytometry
ChIP

Software Mentioned

winMDi
Image J

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.