PMID: 6113592Apr 1, 1981Paper

Depletion of glutathione selectively inhibits synthesis of leukotriene C by macrophages

Proceedings of the National Academy of Sciences of the United States of America
C A RouzerZ A Cohn

Abstract

We have examined the role of glutathione synthesis and intracellular glutathione content in the formation of leukotriene C (LTC) by mouse peritoneal macrophages. For this purpose, we utilized the drug buthionine sulfoximine (BSO), a specific inhibitor of glutathione synthesis. Thirty minutes after the addition of BSO (200 microM) to macrophage cultures, when glutathione synthesis was inhibited approximately 80%, the cells responded to a zymosan challenge with a normal release of LTC. During this period, intracellular glutathione stores were not significantly depleted. Cells exposed to BSO for 2 hr or more exhibited marked decreases in glutathione levels and a progressive inhibition of LTC synthesis. After exposure to BSO for 16 hr, intracellular glutathione was undetectable, and no LTC was synthesized by the cells. Treatment of macrophages with BSO for 16 hr had no effect on cell viability, phagocytosis, total release of arachidonic acid, or prostaglandin synthesis. However, an increased synthesis of hydroxyicosatetraenoic acids in BSO-treated cells compensated for the diminished production of LTC. We conclude that BSO produces a specific, time-dependent inhibition of LTC synthesis as a result of intracellular glutathione deple...Continue Reading

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Citations

May 1, 1993·Molecular Reproduction and Development·M Yoshida
Jan 1, 1990·Pharmacology & Therapeutics·G S AhluwaliaD A Cooney
Jan 1, 1982·International Journal of Immunopharmacology·P DaviesF A Kuehl
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J K Dethmers, A Meister
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J M WilliamsonA Meister
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C A RouzerZ A Cohn
Apr 1, 1982·The Journal of Experimental Medicine·W A ScottZ A Cohn
Mar 1, 1986·The Journal of Experimental Medicine·A O FelsZ A Cohn
Mar 1, 1987·The Journal of Experimental Medicine·H Watanabe, S Bannai
Jan 1, 1994·The Journal of Experimental Medicine·R M Steinman, C L Moberg
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Jan 9, 2015·Nature·Michelle L ReniereDaniel A Portnoy
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Apr 1, 2018·Arhiv za higijenu rada i toksikologiju·Kristina Dominko, Domagoj Đikić
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Dec 16, 2006·Archives of Biochemistry and Biophysics·Sergio F MartínYusuf A Hannun

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