Depletion of intracellular calcium stores activates a calcium conducting nonselective cation current in mouse pancreatic acinar cells.

The Journal of Biological Chemistry
E KrauseI Schulz

Abstract

Receptor-mediated Ca2+ release from inositol (1,4,5)-trisphosphate (IP3)-sensitive Ca2+ stores causes "capacitative calcium entry" in many cell types (Putney, J. W., Jr. (1986) Cell Calcium 7, 1-12; Putney, J. W., Jr. (1990) Cell Calcium 11, 611-624). We used patch-clamp and fluorescence techniques in isolated mouse pancreatic acinar cells to identify ion currents and cytosolic calcium concentrations under conditions in which intracellular Ca2+ stores were emptied. We found that depletion of Ca2+ stores activated a calcium-release-activated nonselective cation current (ICRANC) which did not discriminate between monovalent cations. ICRANC possessed a significant conductance for Ca2+ and Ba2+. It was not inhibited by La3+, Gd3+, Co2+, or Cd2+ but was completely abolished by flufenamic acid or genistein. In whole cell and cell-attached recordings, a 40-45 pS nonselective cation channel was identified which was activated by Ca2+ store depletion. Calcium entry as detected by single cell fluorescence measurements with fluo-3 or fura-2, showed the same pharmacological properties as ICRANC. We conclude that in mouse pancreatic acinar cells 40-45 pS nonselective cation channels serve as a pathway for capacitative Ca2+ entry. This entry ...Continue Reading

References

Nov 11, 1990·The American Journal of Physiology·L J GreenfieldJ Linden
Nov 11, 1990·Cell Calcium·J W Putney
Feb 1, 1986·Cell Calcium·J W Putney
Nov 1, 1989·The Journal of Physiology·G MatthewsR Penner
Nov 30, 1983·Biochemical and Biophysical Research Communications·D L OchsJ A Williams
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Nov 15, 1995·The Biochemical Journal·M J Berridge
May 1, 1995·The Journal of Physiology·A Schmid, I Schulz
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C FasolatoR Penner
Aug 1, 1993·Pflügers Archiv : European journal of physiology·B NiliusG Droogmans
Jan 15, 1995·The Biochemical Journal·Y Hu, W P Schilling
Oct 1, 1994·Pflügers Archiv : European journal of physiology·D FranziusR Penner
Jul 1, 1994·Biophysical Journal·A Lückhoff, D E Clapham
Oct 1, 1994·The American Journal of Physiology·L Vaca, D L Kunze
Nov 1, 1994·The American Journal of Physiology·L VacaW P Schilling
Aug 15, 1994·Biochemical and Biophysical Research Communications·D I YuleJ A Williams
Jun 1, 1994·Pflügers Archiv : European journal of physiology·E C Toescu, O H Petersen
Mar 1, 1994·Trends in Pharmacological Sciences·C FasolatoT Pozzan
Jun 1, 1993·The Journal of Physiology·M Hoth, R Penner
Jan 1, 1994·The American Journal of Physiology·E NiggliW J Lederer
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Zweifach, R S Lewis

❮ Previous
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Citations

Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W Putney, R R McKay
Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·O H PetersenA V Tepikin
Feb 10, 2000·Archives of Biochemistry and Biophysics·J R NoferM Tepel
Sep 1, 2005·Pflügers Archiv : European journal of physiology·James W Putney
Sep 1, 2006·Pflügers Archiv : European journal of physiology·Anant B Parekh
Aug 3, 2005·Cellular and Molecular Neurobiology·Zhengchang SuJ Edwin Blalock
Apr 13, 2010·Biochemical Pharmacology·Javier MilaraJulio Cortijo
Jun 18, 2004·Cell Calcium·Aldebaran M HoferSilvana Curci
Feb 14, 2002·Biochemical Pharmacology·C Camello-AlmarazP J Camello
Sep 7, 2001·Trends in Pharmacological Sciences·V Sorrentino, R Rizzuto
Mar 22, 2002·British Journal of Pharmacology·Kuo-Jung LoSheau-Huei Chueh
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia V GerasimenkoOle H Petersen
May 5, 2007·Journal of Cell Science·James W Putney
Sep 24, 1999·Biological Chemistry·I SchulzA Schmid
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Trevor J Shuttleworth
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·G VazquezJ W Putney
Jul 1, 2008·Expert Opinion on Drug Discovery·Isabella DerlerChristoph Romanin
Jul 27, 1999·The Journal of Physiology·J WarnatA Cavalié
Jul 31, 2013·The Journal of Physiology·Julia V GerasimenkoOle H Petersen
Oct 12, 2010·Cell Calcium·Alexey ShalyginElena Kaznacheyeva
Sep 9, 2011·IUBMB Life·Claudia Moreno, Luis Vaca
Nov 5, 2002·Nature Cell Biology·Kartik VenkatachalamDonald L Gill
Apr 27, 2005·Biochemical and Biophysical Research Communications·Kun-Ta YangKe-Li Tsai
Dec 9, 2008·Biochimica Et Biophysica Acta·Ginés M SalidoJuan A Rosado
Feb 5, 2002·BMC Physiology·Mark Estacion, William P Schilling
May 1, 2016·Biochimica Et Biophysica Acta·Jose J LopezJuan A Rosado
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·C Montell
May 16, 1998·The Journal of Biological Chemistry·K A FaganD M Cooper

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