PMID: 9542530Jul 1, 1997Paper

Depletion of mitochondrial DNA by ddC in untransformed human cell lines

Somatic Cell and Molecular Genetics
I NelsonA E Harding

Abstract

In order to study the interaction between the nuclear and mitochondrial genomes we have developed a non-transformed cell system. It is based upon the complete removal of mtDNA from fibroblasts by treatment with a nucleoside analogue, 2',3' dideoxycytidine (ddC). After exposure to ddC we were able to generate viable fibroblasts devoid of mtDNA and to successfully repopulate them with exogenous mitochondria. This model system will be useful in characterizing nuclear mitochondrial interactions and in studying the effects of different nuclear backgrounds on the expression of different primary defects of mtDNA associated with human disease.

References

Mar 1, 1986·Somatic Cell and Molecular Genetics·P DesjardinsR Morais
Jan 1, 1995·Life Sciences·M MagnaniL Rossi
Jun 1, 1994·Journal of Bioenergetics and Biomembranes·D C Wallace

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Citations

May 30, 2001·Advanced Drug Delivery Reviews·I E Scheffler
May 20, 2008·QJM : Monthly Journal of the Association of Physicians·A M HallM R Duchen
May 6, 2015·International Journal of Molecular Sciences·Susanna SchubertPeter Seibel
Feb 5, 2004·Reproductive Biomedicine Online·Justin C St JohnShahinaz El Shourbagy
Dec 3, 2014·Redox Biology·Heather M WilkinsRussell H Swerdlow
Jan 24, 2007·Journal of Neuroscience Research·Russell H Swerdlow
Apr 17, 2008·Journal of Bioenergetics and Biomembranes·Christophe RocherThierry Letellier
Aug 11, 2006·American Journal of Physiology. Cell Physiology·Shaharyar M KhanRussell H Swerdlow
Sep 2, 2020·Scientific Reports·Emma R DawsonMichael A Teitell
Dec 14, 2018·Cancer Metastasis Reviews·Thomas C BeadnellDanny R Welch
May 14, 2017·G3 : Genes - Genomes - Genetics·Kanokwan Srirattana, Justin C St John

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