Depletion of mitochondrial DNA stabilizes C1qTNF-related protein 6 mRNA in muscle cells.

Journal of Korean Medical Science
Mi-Jin KimSeung-Yoon Park

Abstract

Mutation and reduction of mitochondrial DNA (mtDNA) have been suggested as factors in the pathogenesis of several metabolic diseases. Recently, we demonstrated that C1qTNF-related protein-6 (CTRP6) is involved in fatty acid metabolism in muscle cells. In this study, we showed that expression of CTRP6 was up-regulated in mtDNA-depleted C2C12 cells, which displayed a marked decrease in cellular mtDNA and ATP content. Replacement of mtDNA normalized the expression level of CTRP6 similar to that in normal C2C12 cells, indicating that CTRP6 expression was up-regulated by mtDNA depletion. However, CTRP6 promoter activity remained unchanged in mtDNA-depleted cells. We also found that mtDNA depletion inhibited decay of CTRP6 mRNA. Taken together, mtDNA depletion induces an increase in CTRP6 expression by increasing mRNA stability.

References

May 1, 1985·Molecular and Cellular Biology·P DesjardinsR Morais
Aug 28, 1969·Journal of Molecular Biology·E ZylberS Penman
Jul 21, 2000·The Journal of Clinical Investigation·G I Shulman
Feb 17, 2001·Molecular Biology of the Cell·C B EpsteinR A Butow
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eva TomasNeil B Ruderman
Jan 20, 2004·Trends in Biochemical Sciences·David Carling
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Inmaculada García-RuizJosé A Solís-Herruzo
Feb 29, 2008·Experimental & Molecular Medicine·Wan LeeSeung Yoon Park
Jul 24, 2010·Molecules and Cells·Mi-Jin KimSeung-Yoon Park

❮ Previous
Next ❯

Citations

Feb 3, 2015·Cell Biology International·Wen-jing WuGong-she Yang
Apr 30, 2016·Acta Biochimica Et Biophysica Sinica·Wenjing WuGongshe Yang
Oct 31, 2020·BioMed Research International·Xin ZhuXin Gou
May 26, 2017·Journal of Agricultural and Food Chemistry·Wenjing WuGongshe Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.