Depletion of natural killer cells does not result in neurologic disease due to Sarcocystis neurona in mice with severe combined immunodeficiency

The Journal of Parasitology
Debra C SellonJ B Dame

Abstract

Sarcocystis neurona is an apicomplexan parasite that is the primary etiologic agent of equine protozoal myeloencephalitis in horses. Protective immune responses in horses have not been determined, but interferon-gamma (IFN-gamma) is considered critical for protection from neurologic disease in mice. The role of adaptive and innate immune responses in control of parasites was explored by infecting BALB/c, IFN-gamma knockout (GKO), and severe combined immune deficient (SCID) mice with S. neurona (10(4) sporocysts/mouse). Immune competent BALB/c mice eliminated parasites within 30 days, with no sign of neurologic disease, whereas GKO mice developed fulminant neurologic disease. In contrast, SCID mice remained healthy throughout the experimental period despite the persistence of parasite at low levels in some mice. Treatment with anti-IFN-gamma antibody resulted in neurologic disease in infected SCID mice. Although SCID mice lack adaptive immune responses, they have natural killer (NK) cells capable of producing significant quantities of IFN-gamma. Therefore, SCID mice were infected with sporocysts of S. neurona and treated with anti-asialo GM1. Depletion of NK cells, confirmed by flow cytometry, did not result in neurologic diseas...Continue Reading

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Citations

Nov 13, 2004·Clinical and Diagnostic Laboratory Immunology·Debra C SellonJohn B Dame
Nov 1, 2008·The Journal of Parasitology·Sharon G WitonskyDavid S Lindsay
Dec 3, 2014·The Veterinary Clinics of North America. Equine Practice·Daniel K HoweStephen M Reed
Jun 23, 2005·Veterinary Parasitology·Thomas MullaneyLinda S Mansfield
Aug 20, 2015·Stem Cells Translational Medicine·Dachun WangRick A Wetsel
Jan 1, 2014·Journal of Veterinary Medicine·S Rochelle LewisSharon G Witonsky
Sep 3, 2021·Veterinary Immunology and Immunopathology·Alayna N HayCaroline Leeth

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Methods Mentioned

BETA
PCR
protein assay
light microscopy
electrophoresis
flow cytometry

Software Mentioned

SigmaStat
SPSS

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