Abstract
Previously, we have shown that paraspeckle protein 1 (PSPC1), a protein component of paraspeckles that was involved in cisplatin-induced DNA damage response (DDR), probably functions at the G1/S checkpoint. In the current study, we further examined the role of PSPC1 in another DNA-damaging agent, methyl methanesulfonate (MMS)-induced DDR, in particular, focusing on MMS-induced apoptosis in HeLa cells. First, it was found that MMS treatment induced the expression of PSPC1. While MMS treatment alone can induce apoptosis, depletion of PSPC1 expression using siRNA significantly increased the level of apoptosis following MMS exposure. In contrast, overexpressing PSPC1 decreased the number of apoptotic cells. Interestingly, morphological observation revealed that many of the MMS-treated PSPC1-knockdown cells contained two or more nuclei, indicating the occurrence of mitotic catastrophe. Cell cycle analysis further showed that depletion of PSPC1 caused more cells entering the G2/M phase, a prerequisite of mitosis catastrophe. On the other hand, over-expressing PSPC1 led to more cells accumulating in the G1/S phase. Taken together, these observations suggest an important role for PSPC1 in MMS-induced DDR, and in particular, depletion o...Continue Reading
References
Apr 1, 1992·Molecular & General Genetics : MGG·K AyscoughP Nurse
Aug 1, 1989·Genetics·L MolzD Beach
Nov 15, 1984·Nature·J R McIntosh
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jun 20, 1996·Nature·T WaldmanB Vogelstein
Apr 18, 1997·Cell·O BezzubovaJ M Buerstedde
Sep 12, 1998·Molecular Cell·G FangM W Kirschner
Feb 27, 2001·FEBS Letters·J Bartek, J Lukas
May 22, 2001·Molecular Biology of the Cell·P R AndreassenR L Margolis
Jun 19, 2001·Biochimica Et Biophysica Acta·V A Smits, R H Medema
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·E A Nigg
Jan 16, 2002·Current Biology : CB·Jens S AndersenAngus I Lamond
Jan 16, 2002·Current Biology : CB·Archa H FoxAngus I Lamond
May 7, 2002·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·I B RoninsonB D Chang
Jun 26, 2002·Oncogene·Birgit Spänkuch-SchmittKlaus Strebhardt
Mar 4, 2003·Nature Reviews. Cancer·Yosef Shiloh
May 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xiaoqi Liu, Raymond L Erikson
Jun 7, 2003·Developmental Cell·Florence Margottin-GoguetPeter K Jackson
Apr 13, 2004·Oncogene·Maria CastedoGuido Kroemer
May 14, 2004·Biology of Reproduction·Reiko MyojinYasuyuki Kurihara
Jun 11, 2004·Annual Review of Biochemistry·Aziz SancarStuart Linn
Jul 13, 2005·Nucleic Acids Research·Cecilia LundinThomas Helleday
Feb 14, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yanke YuJun Yang
Apr 28, 2006·Biology of Reproduction·Sho KuwaharaYasuyuki Kurihara
Dec 19, 2006·Chemical Research in Toxicology·Michael D Wyatt, Douglas L Pittman
May 26, 2007·Science·Shuhei MatsuokaStephen J Elledge
Feb 5, 2008·Cancer Research·Ana Belén Martínez-CruzJesús M Paramio
Apr 12, 2008·Cell Death and Differentiation·H VakifahmetogluB Zhivotovsky
Nov 27, 2008·Cell Biochemistry and Biophysics·Magda Budzowska, Roland Kanaar
Jun 15, 2010·Mutation Research·Wei WuJun Yang
Oct 16, 2010·Toxicology and Industrial Health·Seyed Jalal HosseinimehrSohila Mohammadifar
Dec 15, 2010·DNA Repair·Kyungsoo HaWilliam S Dynan
Apr 30, 2011·Nature Reviews. Molecular Cell Biology·Ilio VitaleGuido Kroemer
Jan 17, 2012·Toxicology and Applied Pharmacology·Mei-Hua HsuSheng-Chu Kuo
Oct 1, 2013·Journal of Medicinal Food·Chien-An ChenYu-Jen Chen
May 14, 2014·PloS One·Xiangjing GaoJun Yang
Aug 8, 2014·Nucleic Acids Research·Shuyi LiWilliam S Dynan