Depolarization induces insulin-like growth factor binding protein-2 expression in vivo via NMDA receptor stimulation

Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
S HolminA C Sandberg Nordqvist

Abstract

The effect of depolarization and N-methyl-D-aspartate (NMDA) receptor blockade on insulin-like growth factor-I (IGF-I), IGF binding protein-2 (IGFBP-2) and IGFBP-4 expression was analysed in vivo. Depolarization was induced in adult rat brains by applying 3 M KCl to the exposed cortex for 10 min. A subgroup of animals also received daily injections of MK-801. Four days after KCl exposure, the brains were analysed by in situ hybridization, immunohistochemistry and TUNEL. A significant upregulation of IGFBP-2 mRNA and protein was detected in astrocytes after KCl exposure This upregulation was reduced by MK-801 treatment. No alterations in IGF-I or IGFBP-4 mRNA levels were noted. We did not detect TUNEL positive cells, morphological signs of necrosis or apoptosis, or neuronal loss in the depolarized zone. Taken together, these findings indicate that upregulation of IGFBP-2 by depolarization is mediated by NMDA receptors, and, as no neuronal damage was detected, astrocytic NMDA receptors may be responsible for this upregulation.

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Citations

Feb 17, 2010·The Journal of Clinical Endocrinology and Metabolism·Jyh-Ming LiouJaw-Town Lin
Jan 12, 2010·The Journal of Clinical Endocrinology and Metabolism·Sandra W K de KortAnita C S Hokken-Koelega
Jul 27, 2007·The Journal of Clinical Endocrinology and Metabolism·Annamaria ColaoGaetano Lombardi
Mar 21, 2012·Nature Reviews. Neuroscience·Ana M Fernandez, Ignacio Torres-Alemán
Sep 6, 2012·Journal of Cellular Physiology·Brian J LeibowitzWendie S Cohick
Dec 7, 2013·Clinical Endocrinology·Yunze XuYiran Huang
Apr 30, 2014·Frontiers in Endocrinology·Andreas HoeflichJulia Brenmoehl
Apr 2, 2013·Cellular and Molecular Life Sciences : CMLS·R C Agis-Balboa, A Fischer

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