PMID: 4907957Feb 1, 1970Paper

Depolarization of the intrinsic and extrinsic fluorescence of pepsinogen and pepsin

The Biochemical Journal
F W Teale, R A Badley

Abstract

1. The effects on the intrinsic tryptophan emission anisotropy of pepsin and pepsinogen solutions produced by (a) changes in temperature, (b) increases in viscosity with added glycerol at constant temperature and (c) decreases in lifetime through collisional quenching by potassium iodide were measured at several excitation wavelengths. The rotational-relaxation times calculated from results provided by method (b) approximate to the theoretical values for the two proteins, on taking hydration and shape factors into account, on the basis of random orientation of the tryptophan groups within the macromolecules. Differences between the results provided by methods (b) and (c) are attributable to inter-tryptophan resonance-energy-transfer depolarization, and the anomalous values recorded in method (a) can be attributed to the temperature-dependence of the limiting anisotropies. 2. Two different monomeric conjugates of pepsin, each containing one extrinsic fluorescent group per macromolecule, gave widely different relaxation times. This difference may arise from a specific orientation of the emission dipole in the enzyme. In active-site-labelled pepsin (1-dimethylaminonaphthalene-5-sulphonylphenylalanine-pepsin) this orientation would...Continue Reading

Citations

Feb 1, 1973·European Journal of Biochemistry·Z Bohak
Oct 13, 2009·Journal of Photochemistry and Photobiology. B, Biology·Atul KumarArchana Pundle
Apr 12, 1983·Biochemistry·J R LakowiczA Balter
Oct 1, 1973·Archives of Biochemistry and Biophysics·R F Chen
Jul 1, 1981·Analytical Biochemistry·M R Eftink, C A Ghiron
Jun 14, 1971·Journal of Molecular Biology·R A Badley, F W Teale
Feb 1, 1975·Archives of Biochemistry and Biophysics·Y A Zagyansky
Jan 1, 1973·International Journal of Peptide and Protein Research·G Jori, N Genov
Oct 1, 1982·European Journal of Biochemistry·K G SteinhäuserJ Dijk
Jan 1, 1980·Journal of Biochemical and Biophysical Methods·J R Lakowicz
Dec 20, 1978·Biochimica Et Biophysica Acta·D C Kramp, I Feldman
Oct 1, 1974·Immunochemistry·Y A Zagyansky, E M Gavrilova
Sep 1, 1987·Archives of Biochemistry and Biophysics·J M ValpuestaJ M Macarulla

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