PMID: 9191076Jun 1, 1997Paper

Deprenyl induces the tyrosine hydroxylase enzyme in the rat dopaminergic nigrostriatal system

Brain Research. Molecular Brain Research
J A Rodríguez-GómezA Machado

Abstract

Chronic treatment of aged rats with deprenyl prevents age-induced protein oxidation in substantia nigra and protects tyrosine hydroxylase (TH) enzyme against inactivation [11]. With these precedents, we treated adult rats with deprenyl for 3 weeks in order to get further insight in the mechanism by which deprenyl exerts such actions. After completing the treatment, dopamine (DA) levels markedly increased in both striatum and substantia nigra while levels of the acid DA metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), decreased in the two brain areas, thus proving MAO-inhibiting properties of the treatment. We then studied the cellular expression of TH mRNA by in situ hybridization. Following treatment with deprenyl, levels of TH mRNA were significantly higher in individual dopaminergic nigral cell bodies than in those of control rats (+74%). Western blotting analysis of TH enzyme amount revealed a positive effect of the treatment in both the terminal field (+44%) and the cell body region (+31%). This correlation between TH mRNA and amount was also extended to TH enzyme activity in the two brain areas studied, which significantly increased in striatum (+57%) and substantia nigra (+35%) following d...Continue Reading

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Citations

Jun 17, 1998·Molecular Neurobiology·J Haavik, K Toska
Dec 13, 2000·Brain Research. Molecular Brain Research·M Romero-RamosA Machado
Feb 26, 2003·Neurobiology of Aging·Hoda KhaldyDarío Acuña-Castroviejo
Jul 2, 2008·Parkinsonism & Related Disorders·P FoleyP Riederer
Nov 16, 2006·Rejuvenation Research·Fariba EsmaeiliSeyed Javad Mowla
Feb 24, 2006·Neurochemistry International·Karuppagounder S SaravananKochupurackal P Mohanakumar
Oct 6, 2005·Brain Research. Molecular Brain Research·Rocío M de PablosJosefina Cano

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