Depth matters: effects of precipitation regime on soil microbial activity upon rewetting of a plant-soil system

The ISME Journal
Ilonka C EngelhardtRomain L Barnard

Abstract

Changes in frequency and amplitude of rain events, that is, precipitation patterns, result in different water conditions with soil depth, and likely affect plant growth and shape plant and soil microbial activity. Here, we used 18O stable isotope probing (SIP) to investigate bacterial and fungal communities that actively grew or not upon rewetting, at three different depths in soil mesocosms previously subjected to frequent or infrequent watering for 12 weeks (equal total water input). Phylogenetic marker genes for bacteria and fungi were sequenced after rewetting, and plant-soil microbial coupling documented by plant 13C-CO2 labeling. Soil depth, rather than precipitation pattern, was most influential in shaping microbial response to rewetting, and had differential effects on active and inactive bacterial and fungal communities. After rewetting, active bacterial communities were less rich, more even and phylogenetically related than the inactive, and reactivated throughout the soil profile. Active fungal communities after rewetting were less abundant and rich than the inactive. The coupling between plants and soil microbes decreased under infrequent watering in the top soil layer. We suggest that differences in fungal and bact...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Aug 17, 2005·FEMS Microbiology Reviews·Wietse de BoerLynne Boddy
Dec 8, 2005·Applied and Environmental Microbiology·Catherine Lozupone, Rob Knight
Apr 21, 2009·Molecular Biology and Evolution·Morgan N PriceAdam P Arkin
Oct 22, 2009·The New Phytologist·Nadine K RuehrRomain L Barnard
Nov 17, 2009·Bioinformatics·J Gregory CaporasoRob Knight
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Apr 17, 2010·Bioinformatics·Steven W KembelCampbell O Webb
Apr 23, 2010·The New Phytologist·Kessy AbarenkovUrmas Kõljalg
Jul 17, 2010·Microbial Ecology·Azadeh BapiriJohannes Rousk
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Arnaud DechesneBarth F Smets
Aug 17, 2010·Bioinformatics·Robert C Edgar
Oct 15, 2010·Mycologia·Ari JumpponenJohn Blair
Mar 3, 2011·Microbial Ecology·Steven J Blazewicz, Egbert Schwartz
Sep 6, 2011·Applied and Environmental Microbiology·David BerryAlexander Loy
Jan 5, 2012·Rapid Communications in Mass Spectrometry : RCM·Susan Q LangGretchen L Früh-Green
Jun 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sarah A PlacellaMary K Firestone
Oct 12, 2012·The ISME Journal·Gang Wang, Dani Or
Jun 5, 2013·FEMS Microbiology Ecology·Roey AngelOsnat Gillor
Jul 5, 2013·The ISME Journal·Romain L BarnardMary K Firestone
Sep 14, 2013·Frontiers in Microbiology·Franciska T de Vries, Ashley Shade
Oct 22, 2013·Bioinformatics·Jiajie ZhangAlexandros Stamatakis
Nov 12, 2014·The New Phytologist·Roland HasibederMichael Bahn
Apr 17, 2015·FEMS Microbiology Ecology·Lluvia Vargas-GastélumMeritxell Riquelme
Mar 5, 2016·The ISME Journal·Ember M MorrisseyBruce A Hungate
Aug 26, 2016·Microbial Ecology·Rodrigo Gouvêa TaketaniItamar Soares Melo
Oct 27, 2016·PeerJ·Torbjørn RognesFrédéric Mahé

❮ Previous
Next ❯

Citations

Oct 28, 2019·Applied and Environmental Microbiology·Hannah KleyerDani Or
Sep 12, 2019·Frontiers in Microbiology·Nobuhiko ShigyoToshihide Hirao
Jan 8, 2021·The ISME Journal·Annelein MeisnerAnders Priemé

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
amplicon sequencing

Software Mentioned

QIIME
picante
PyNAST
FastTree
R
WinRHIZO
VSEARCH
UCLUST
BLAST
Illumina MiSeq Reporter

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.