Depth profiles of pulmonary surfactant protein B in phosphatidylcholine bilayers, studied by fluorescence and electron spin resonance spectroscopy

Biochemistry
A CruzJ Perez-Gil

Abstract

Pulmonary surfactant-associated protein B (SP-B) has been isolated from porcine lungs and reconstituted in bilayers of dipalmitoylphosphatidylcholine (DPPC) or egg yolk phosphatidylcholine (PC) to characterize the extent of insertion of the protein into phospholipid bilayers. The parameters for the interaction of SP-B with DPPC or PC using different reconstitution protocols have been estimated from the changes induced in the fluorescence emission spectrum of the single protein tryptophan. All the different reconstituted SP-B-phospholipid preparations studied had similar Kd values for the binding of the protein to the lipids, on the order of a few micromolar. The depth of penetration of SP-B into phospholipid bilayers has been estimated by the parallax method, which compares the relative efficiencies of quenching of the protein fluorescence by a shallow or a deeper spin-labeled phospholipid probe. SP-B tryptophan was found to be located 10-13 A from the center of bilayers, which is consistent with a superficial location of SP-B in phosphatidylcholine membranes. Parallax experiments, as well as resonance energy transfer from SP-B tryptophan to an acceptor probe located in the center of the bilayer, indicate that there are signifi...Continue Reading

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Citations

Sep 22, 2012·European Biophysics Journal : EBJ·Bárbara OlmedaJesús Pérez-Gil
Sep 13, 2002·Journal of Molecular Biology·Yiannis N KaznessisRonald G Larson
May 23, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·I PlasenciaJ Pérez-Gil
Dec 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shelli L FreyKa Yee C Lee
Sep 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Frank BraschMatthias Ochs
Jun 30, 2011·Nanoscale Research Letters·Elisabete Ms CastanheiraMaria-João Rp Queiroz
Aug 25, 2001·Analytical Biochemistry·I PlasenciaJ Pérez-Gil
Apr 4, 2006·Chemistry and Physics of Lipids·Jörg H Kleinschmidt
Apr 17, 2007·Biophysical Journal·Michael R MorrowKevin M W Keough
Jun 5, 2012·Biochimica Et Biophysica Acta·Cristina Casals, Olga Cañadas
Mar 1, 2016·Biochimica Et Biophysica Acta·Svetlana Baoukina, D Peter Tieleman
Jun 11, 2004·Biophysical Journal·Vincent Schram, Stephen B Hall
Oct 17, 2003·Biochimica Et Biophysica Acta·Vincent SchramStephen B Hall
Oct 13, 2000·Biochimica Et Biophysica Acta·E J Veldhuizen, H P Haagsman
Dec 11, 2007·Biochimica Et Biophysica Acta·Jesus Ayala-SanmartinJacques Gallay
Sep 28, 2014·Chemistry and Physics of Lipids·Elisa Parra, Jesús Pérez-Gil
Mar 16, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Marta Martínez-CalleJesús Pérez-Gil
Mar 24, 2021·Archives of Biochemistry and Biophysics·José Carlos Castillo-SánchezJesús Pérez-Gil
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp

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