Depuration kinetics of murine norovirus in shellfish

Food Research International
David PoloJesús L Romalde

Abstract

This study evaluates and compares the uptake rates and depuration kinetics of murine norovirus (MNV-1), as a human norovirus surrogate, in Manila clams (Venerupis philippinarum) and Mediterranean mussels (Mytilus galloprovincialis). Ten trials of 70kg/trial (five with each mollusk) were performed. Mollusks were subjected to a controlled bioaccumulation step of 24h with 102pfu MNV-1/mL seawater. Then, mollusks were relocated in an experimental depuration system for 7days. Viral contamination was quantified after bioaccumulation and then daily during depuration by reverse transcription-real time PCR (RT-qPCR) with TaqMan probes. Infectivity assays were conducted to test the presence of infectious viral particles at the end of the depuration period. Results showed significant differences in the uptake and removal viral rates between molluscan species. The average viral uptake for clams and mussels were 5.4×106 and 4.0×105RNA copies/g digestive tissue respectively, representing an uptake rate >90% higher in clams. The average reductions with regard to the initial levels were 60.5% for clams and 91.6% for mussels. On the other hand, a similar logarithmic trend line in MNV-1 depuration kinetics was observed in both bivalves, with two...Continue Reading

Citations

Nov 7, 2019·Food and Environmental Virology·Maria Grazia AmorosoGiovanna Fusco
Mar 18, 2021·Food and Environmental Virology·Maria Grazia AmorosoGiovanna Fusco
Jul 7, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Andrew D YoungerMickael Teixeira Alves

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