Depurination of yeast 26S ribosomal RNA by recombinant ricin A chain

Biochemical and Biophysical Research Communications
J L BradleyP M McGuire

Abstract

The nature of the modification of yeast ribosomes by the recombinant form of the ricin A chain has been examined. Evidence is presented that the 26S rRNA molecule is depurinated at a specific site and that the activity is inhibited by antibody raised to ricin A chain. It thus appears that the recombinant form of this toxin retains the depurination activity of the native molecule. These results are consistent with the model that the site of depurination is in a highly conserved sequence forming a loop on the surface of the ribosome, a domain involved in elongation factor-dependent binding of aminoacyl-tRNA.

References

Jun 12, 1979·Biochemistry·D B CawleyL L Houston
Jun 1, 1975·European Journal of Biochemistry·L CarrascoD Vazquez
Feb 9, 1976·Biochemical and Biophysical Research Communications·S J MitchellL L Houston
Jan 1, 1986·Applied and Environmental Microbiology·K M Dombek, L O Ingram
Apr 15, 1985·European Journal of Biochemistry·F I LambJ M Lord
Jul 31, 1985·Biochemical and Biophysical Research Communications·T G ObrigR J Colinas
May 1, 1985·Journal of Natural Products·A Gasperi-CampaniF Stirpe
Dec 30, 1968·Biochemical and Biophysical Research Communications·P PhilippsenH G Zachau
Aug 1, 1984·Archives of Biochemistry and Biophysics·M S EllerP M McGuire
Jan 1, 1982·Immunological Reviews·F K JansenG A Voisin
Dec 21, 1981·Nucleic Acids Research·G M VeldmanJ P Ebel
Jan 1, 1981·Pharmacology & Therapeutics·S Olsnes, A Pihl

❮ Previous
Next ❯

Citations

Jan 1, 1988·Progress in Biophysics and Molecular Biology·H A RauéW Musters
Jan 1, 1992·Pharmacology & Therapeutics·J D Irvin, F M Uckun
Mar 17, 1999·International Journal of Biological Macromolecules·F J Lebeda, M A Olson
May 14, 2005·Expert Review of Vaccines·Catherine J MarsdenJ Michael Lord
Nov 10, 2011·Toxins·Björn Becker, Manfred J Schmitt
Dec 21, 1993·Biochimica Et Biophysica Acta·L BarbieriF Stirpe
Apr 28, 1989·Biochemical and Biophysical Research Communications·S RamakrishnanF J Xu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.