Deregulated expression of cryptochrome genes in human colorectal cancer

Molecular Cancer
G MazzoccoliA Piepoli

Abstract

Circadian disruption and deranged molecular clockworks are involved in carcinogenesis. The cryptochrome genes (CRY1 and CRY2) encode circadian proteins important for the functioning of biological oscillators. Their expression in human colorectal cancer (CRC) and in colon cancer cell lines has not been evaluated so far. We investigated CRY1 and CRY2 expression in fifty CRCs and in the CaCo2, HCT116, HT29, SW480 cell lines. CRY1 (p = 0.01) and CRY2 (p < 0.0001) expression was significantly changed in tumour tissue, as confirmed in a large independent CRC dataset. In addition, lower CRY1 mRNA levels were observed in patients in the age range of 62-74 years (p = 0.018), in female patients (p = 0.003) and in cancers located at the transverse colon (p = 0.008). Lower CRY2 levels were also associated with cancer location at the transverse colon (p = 0.007). CRC patients displaying CRY1 (p = 0.042) and CRY2 (p = 0.043) expression levels over the median were hallmarked by a poorer survival rate. Survey of selected colon cancer cell lines evidenced variable levels of cryptochrome genes expression and time-dependent changes in their mRNA levels. Moreover, they showed reduced apoptosis, increased proliferation and different response to 5-f...Continue Reading

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Citations

Feb 23, 2017·International Journal of Cancer. Journal International Du Cancer·Ali MteyrekFrancis Lévi
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Aug 28, 2021·Biomedicines·María I Aroca-SiendonesJosefa León

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Methods Mentioned

BETA
Assay
electrophoresis
X-ray
transfection
flow cytometry
Fluorescence

Software Mentioned

SDS
SPSS Statistical Package ( SPSS
MATLAB
FACSuite

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