Deregulated expression of promyelocytic leukemia zinc finger protein in B-cell chronic lymphocytic leukemias does not affect cyclin A expression

The Hematology Journal : the Official Journal of the European Haematology Association
Antonio ParradoR A Padua

Abstract

The promyelocytic leukemia zinc finger (PLZF) gene encodes a transcription factor expressed in myeloid, lymphoid and CD34(+) progenitor cells. Structurally related to BCL-6, which is involved in human lymphoma, PLZF may have a role in proliferation, differentiation and survival of hematopoietic cells, that could be mediated by transcriptional repression of the cyclin A gene. Quantitative competitive reverse transcription-polymerase chain reaction was used to measure the levels of expression of PLZF and cyclin A in normal leukocyte subsets (including CD19(+) lymphocytes, n=21) and malignant B lymphocytes (including B-chronic lymphocytic leukemias [B-CLL], n=63). Results obtained with this method were confirmed by Western and Northern blot analysis. Transactivation assays were performed using an expression construct for PLZF and two cyclin A promoter luciferase reporters in an Epstein-Barr virus (EBV)-transformed B-cell line. Cyclin A expression, cell growth kinetics, and cell cycle were analysed in stable clones of the Burkitt lymphoma (BL) B-cell line DG75 with inducible expression of PLZF, generated using the tetracycline-regulated expression system. Expression of PLZF was 100-fold downregulated in 90% B-CLL (56/63) compared t...Continue Reading

Citations

Feb 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Antonio ParradoM Rocío Alvarez-López
Jun 24, 2008·Nature Cell Biology·Catherine LabbayeCesare Peschle
Apr 13, 2004·Oncogene·Federica FelicettiAlessandra Carè
Dec 4, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Sara ContenteRobert M Friedman
Mar 3, 2011·Biochimica Et Biophysica Acta·Hoi Chin HewKiyotsugu Yoshida

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