Deregulation of ARID1A, CDH1, cMET and PIK3CA and target-related microRNA expression in gastric cancer

Oncotarget
Maider Ibarrola-VillavaAndrés Cervantes

Abstract

Genetic and epigenetic alterations play an important role in gastric cancer (GC) pathogenesis. Aberrations of the phosphatidylinositol-3-kinase signaling pathway are well described. However, emerging genes have been described such as, the chromatin remodeling gene ARID1A. Our aim was to determine the expression levels of four GC-related genes, ARID1A, CDH1, cMET and PIK3CA, and 14 target-related microRNAs (miRNAs). We compared mRNA and miRNA expression levels among 66 gastric tumor and normal adjacent mucosa samples using quantitative real-time reverse transcription PCR. Moreover, ARID1A, cMET and PIK3CA protein levels were assessed by immunohistochemistry (IHC). Finally, gene and miRNAs associations with clinical characteristics and outcome were also evaluated. An increased cMET and PIK3CA mRNA expression was found in 78.0% (P = 2.20 × 10-5) and 73.8% (P = 1.00 × 10-3) of the tumors, respectively. Moreover, IHC revealed that cMET and PIK3CA expression was positive in 63.6% and 87.8% of the tumors, respectively. Six miRNAs had significantly different expression between paired-samples, finding five up-regulated [miR-223-3p (P = 1.65 × 10-6), miR-19a-3p (P = 1.23 × 10-4), miR-128-3p (P = 3.49 × 10-4), miR-130b-3p (P = 1.00 × 10-3...Continue Reading

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Citations

Jan 23, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yong-Qiang WangHua Jiang
Aug 20, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keerakarn SomsuanVisith Thongboonkerd
Dec 9, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Onder OzcanOzgur Ilhan Celik
Jul 20, 2016·Journal of Gastric Cancer·Si-Hyong JangMoon Soo Lee
Jul 17, 2018·Oncology Letters·Shiqing LiQingsong Liu
Feb 13, 2019·Molecular Medicine Reports·Huan ZhouXiangtao Lin
Jul 2, 2016·Journal of Experimental & Clinical Cancer Research : CR·Jianwei TianXiaoyan Bai
Feb 13, 2021·Frontiers in Oncology·Xinxin ZhouTeng Ma
Jun 5, 2021·Mutation Research. Reviews in Mutation Research·Ruihua WangKaren Poon

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Methods Mentioned

BETA
reverse transcription PCR
PCR
light microscopy

Software Mentioned

DIANA
GraphPad Prism
RQ Manager
Target
SPSS
scan

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