Derivation and characterization of progenitor stem cells from canine allantois and amniotic fluids at the third trimester of gestation

Placenta
R A FernandesMaria Angelica Miglino

Abstract

Fetal tissues are frequently discarded before (amniocentesis) or after birth, which both facilitates stem cell access and helps to overcome ethical concerns. In the present study, we aimed to isolate and characterize stem cells from the allantoic and amniotic fluids (ALF; AMF) of third trimester canine fetuses. This gestation age has not been previously explored for stem cells isolation. The gestational age, cell culture conditions and method of isolation used in this study allowed for the establishment and efficient expansion of ALF and AMF cells. We showed that the majority of ALF and ALF cells express the stem cell markers, such as vimentin, nestin and cytokeratin 18 (CK18). Under appropriate culture conditions AMF derived cells can undergo differentiation into osteogenic, adipogenic, chondrogenic and neuron-like lineages. ALF derived cells showed adipogenic, and chondrogenic potential. Therefore, ALF and AMF cells derived at the third gestation trimester can be qualified as progenitor stem cells, accordingly referred as (alantoic fluid progenitor/stem) ALF PS cells and (amniotic fluid progenitor/stem) AMF PS cells.

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Citations

Sep 29, 2015·Research in Veterinary Science·Hoo Mun-FunNorshariza Nordin
Feb 24, 2015·Reproduction in Domestic Animals = Zuchthygiene·E IaconoB Merlo
Jan 8, 2015·Frontiers in Pharmacology·Christopher D PoradaGraça Almeida-Porada
Oct 30, 2015·Reproduction in Domestic Animals = Zuchthygiene·P O FavaronM A Miglino
Sep 6, 2013·Biology of Reproduction·Jill L SeabrookQuinton A Winger
Mar 7, 2017·Reproductive Biology·Adila A HamidNorshariza Nordin
Jul 15, 2017·Stem Cells and Development·Debora KehlBenedikt Weber
Jun 18, 2017·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Stefano Da SaccoSargis Sedrakyan
Oct 11, 2017·Cell Proliferation·A B TrindadeL C Smith
Oct 9, 2020·In Vitro Cellular & Developmental Biology. Animal·Jieting HuangZhengwei Yuan
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Eleonora IaconoBarbara Merlo
Aug 28, 2021·Frontiers in Cell and Developmental Biology·Christopher StemGraça Almeida-Porada

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