Derivation efficiency, cell proliferation, freeze-thaw survival, stem-cell properties and differentiation of human Wharton's jelly stem cells

Reproductive Biomedicine Online
Chui-Yee FongAriff Bongso

Abstract

Human mesenchymal stem cells (MSC) are non-controversial multipotent stem cells. Their presence in umbilical cord blood (UCB) has been debated in some studies and others report low counts per cord blood unit and poor proliferation rates. On the other hand, Wharton's jelly of human umbilical cords appears to be a rich source of human MSC. This study derived 13 human Wharton's jelly stem cell (WJSC) lines from 13 human umbilical cords (100%) and recovered 4.7 +/- 0.2 x 10(6) live WJSC/cm of cord before culture. Complex culture medium produced greater proliferation rates of the WJSC in culture compared with simple medium. The mean population doubling times were 24.47 +/- 0.33 to 26.25 +/- 0.50 h in complex medium. The stem-cell markers of the WJSC were retained for at least 10 passages in both media. After programmed machine freezing, the thaw-survival rates of WJSC were 85-90% and they could be differentiated into neurons. Given the high derivation efficiency, availability of large numbers of fresh live cells, high expansion capabilities, prolonged maintenance of stem-cell properties and differentiation potential, it is proposed that human WJSC may be frozen at the same time as UCB in cord blood banks for regenerative medicine pu...Continue Reading

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Citations

Dec 18, 2013·Stem Cells and Development·Déborah Afonso CornélioSilvia Regina Batistuzzo de Medeiros
Aug 8, 2014·Journal of Human Genetics·Clara Y CheongWalter Stünkel
Dec 24, 2010·Regenerative Medicine·Limin WangMichael S Detamore
Jun 2, 2012·Stem Cells and Development·Joery De KockVera Rogiers
Sep 17, 2013·Journal of Cellular Biochemistry·Chui-Yee FongAriff Bongso
Dec 12, 2012·Journal of Tissue Engineering and Regenerative Medicine·Noor Hayaty Abu KasimZeti Adura Che Ab Aziz
Dec 27, 2011·Reproductive Biomedicine Online·Kalamegam GauthamanAriff Bongso
Nov 23, 2013·Journal of Cellular Biochemistry·Chui-Yee FongAriff Bongso
Nov 23, 2013·Journal of Cellular Biochemistry·Kimberley TamChui-Yee Fong
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Jan 27, 2015·Cell Transplantation·Dah-Ching DingShinn-Zong Lin
Jul 29, 2010·Journal of Cellular Biochemistry·Chui-Yee FongAriff Bongso
Apr 29, 2015·Molecular Endocrinology·Roy JosephWalter Stünkel
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Dec 12, 2018·Stem Cell Research & Therapy·Ioannis ChristodoulouVassilis Zoumpourlis

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