Derivation of insertin

Cell Motility and the Cytoskeleton
A TeubnerA Wegner

Abstract

Insertin is an actin-binding protein that has been isolated from chicken gizzard smooth muscle that has been shown to be highly homologous to amino acids 962-1292 of tensin [Weigt et al., 1992]. Because of the high homology, we investigated the question whether the mRNAs of insertin and of tensin are derived from the same gene by alternative splicing, whether insertin and tensin are encoded by two different genes, or whether insertin is a proteolytic fragment of tensin. In a Northern blot analysis, mRNA from chicken gizzard was hybridized with oligonucleotides specific for tensin and for the insertin domain of tensin. The tensin-specific oligonucleotide hybridized only with the previously reported 8- and 10-kbp RNAs. However, the insertin domain-specific oligonucleotide hybridized with a 1.2 and a 1.6 kbp RNA in addition to the 8 and 10 kbp RNA. The 1.2- and 1.6-kbp RNA occurred in small amounts, as compared with the 8- and 10-kbp RNA. Southern blot analysis of DNA cleaved by the restriction endonucleases BamH1 and HindIII demonstrated that only one gene for the insertin and tensin exists. Insertin isolated from chicken gizzard smooth muscle was investigated by mass spectrometry. The N-termini of three isolated peptides were fo...Continue Reading

References

Sep 20, 1992·Journal of Molecular Biology·C WeigtH E Meyer
Feb 1, 1991·Journal of Muscle Research and Cell Motility·A Gaertner, A Wegner
Mar 1, 1986·The Journal of Cell Biology·J A Wilkins, S Lin
Nov 1, 1995·The Journal of Cell Biology·S MiyamotoK M Yamada

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Citations

Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Alice Y Welch, Ira M Herman

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