PMID: 6412427Jul 1, 1983Paper

Derivation of monoclonal antibodies against Brucella abortus antigens

Veterinary Immunology and Immunopathology
P J HolmanG G Wagner

Abstract

In vivo immunization, fusion, antibody detection, and cryopreservation procedures for monoclonal antibody production against antigens of Brucella abortus are described. Splenocytes from BALB/c mice immunized with irradiated B. abortus S2308 were fused with Sp2/O-Ag14 myeloma cells and 61 hybridomas secreting anti-Brucella antibodies were cloned. Hybridoma antibody synthesis was detected effectively and most efficiently by enzyme-linked immunosorbent assays. Antibodies from clones of hybridoma A23 reacted with S19 and S2308 whole bacterial cells, while hybridoma B49 reacted primarily with alkali--treated lipopolysaccharides of S19, S1119.3 and S2308. Cryopreservation of clones had no major effect on antibody synthesis. The application of monoclonal anti-Brucella antibodies in the differential diagnosis of bovine brucellosis is discussed.

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Citations

Oct 1, 1984·European Journal of Clinical Microbiology·R A Polin
Aug 3, 1987·Journal of Immunological Methods·S R SamoilovichA J Macario
Feb 1, 1985·Veterinary Immunology and Immunopathology·P G BundesenD B Rylatt
Sep 1, 1985·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·I Greiser-WilkeK Rauter
Oct 1, 1989·Journal of Clinical Microbiology·D A Palmer, J T Douglas
Sep 1, 1987·Annales De L'Institut Pasteur. Microbiology·I Greiser-Wilke, V Moennig

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