Derivation of neurons with functional properties from adult limbal epithelium: implications in autologous cell therapy for photoreceptor degeneration

Stem Cells
Xing ZhaoIqbal Ahmad

Abstract

The limbal epithelium (LE), a circular and narrow epithelium that separates cornea from conjunctiva, harbors stem cells/progenitors in its basal layer that regenerate cornea. We have previously demonstrated that cells in the basal LE, when removed from their niche and cultured in reduced bond morphogenetic protein signaling, acquire properties of neural progenitors. Here, we demonstrate that LE-derived neural progenitors generate neurons with functional properties and can be directly differentiated along rod photoreceptor lineage in vitro and in vivo. These observations posit the LE as a potential source of neural progenitors for autologous cell therapy to treat photoreceptor degeneration in age-related macular degeneration and retinitis pigmentosa.

References

May 1, 1992·Developmental Biology·J D ZieskeH T Keutmann
Jun 1, 1983·The American Journal of Anatomy·R W Clough, J F Rodriguez-Sierra
Jan 10, 1997·Cell·A Hemmati-Brivanlou, D Melton
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·T FurukawaC L Cepko
Sep 12, 1997·Science·F W Pfrieger, B A Barres
Jan 8, 2000·The Journal of Biological Chemistry·A KimuraT Shinohara
Mar 17, 2000·Science·V TropepeD van der Kooy
Feb 7, 2001·Biochemical and Biophysical Research Communications·I AhmadH Pham
Jul 13, 2000·The New England Journal of Medicine·R J TsaiJ K Chen
Jul 13, 2000·The New England Journal of Medicine·I R Schwab, R R Isseroff
Nov 9, 2000·Journal of Neurophysiology·Z H Pan, H J Hu
Feb 7, 2001·Science·E M UllianB A Barres
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PellegriniM De Luca
Jun 8, 2001·Neuron·F KawaiE Miyachi
Nov 6, 2001·Nature Genetics·A J MearsA Swaroop
Sep 19, 2002·Biochemical and Biophysical Research Communications·Xing ZhaoIqbal Ahmad
May 27, 2003·Investigative Ophthalmology & Visual Science·Sumitra BhattacharyaIqbal Ahmad
Jul 10, 2003·Trends in Neurosciences·Larysa Pevny, Mahendra S Rao
Nov 12, 2003·Survey of Ophthalmology·Martin GrueterichScheffer C G Tseng
Mar 24, 2004·Seminars in Cell & Developmental Biology·Iqbal AhmadXing Zhao
Apr 27, 2004·Experimental Eye Research·Robert M LavkerTung-Tien Sun
Jan 8, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Xing ZhaoIqbal Ahmad
Apr 5, 2005·Seminars in Ophthalmology·Ani M DasIqbal Ahmad
May 14, 2005·EMBO Reports·Alena Shkumatava, Carl J Neumann
Jul 30, 2005·Experimental Eye Research·Ursula Schlötzer-Schrehardt, Friedrich E Kruse
Oct 27, 2005·Investigative Ophthalmology & Visual Science·Philip J GageTord Hjalt
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Masayuki AkimotoAnand Swaroop
May 3, 2006·Genes & Development·Olena V TaranovaLarysa H Pevny
Nov 10, 2006·Nature·R E MacLarenR R Ali
Jan 11, 2007·Cell Research·Wei LiScheffer C G Tseng
Feb 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Enrique Salero, Mary E Hatten

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Citations

Jun 18, 2011·British Medical Bulletin·Nick Di Girolamo
May 23, 2014·Visual Neuroscience·Carla B MelloughMajlinda Lako
Jun 10, 2010·Expert Opinion on Biological Therapy·Shu-Zhen WangWeiming Mao
Oct 1, 2011·Current Eye Research·Mikk PauklinDaniel Meller
Dec 4, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Lili TakácsGyörgy Vereb
Mar 4, 2017·Clinical & Experimental Ophthalmology·Samuel McLenachanFred K Chen
Jan 1, 2014·Journal of Clinical Medicine·Fred K ChenDavid A Mackey
Sep 27, 2014·World Journal of Stem Cells·Jinny J YoonTrevor Sherwin

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