Derivatization in capillary electrophoresis

Journal of Chromatography. a
H A BardelmeijerW J Underberg

Abstract

In recent years capillary electrophoresis (CE) has been developed into a versatile separation technique, next to gas and liquid chromatography (LC), well suited for the determination of a wide variety of e.g., pharmaceutical, biomedical and environmental samples. The main advantages of CE over chromatographic separation techniques are its simplicity and efficiency. It is well recognized, however, that the sensitivity and selectivity of the detection are relatively weak points of CE. One way to overcome these limitations is the conversion (derivatization) of the analytes into product(s) with more favourable detection characteristics. Although, in principle, almost any detection mode can be combined with a derivatization procedure, in practice, fluorescence monitoring is favoured in most cases. This paper aims to give a short overview on the various reagents that can be used for pre-, post- and on-column derivatization in CE. First, a short introduction is given on CE as an analytical technique, followed by a discussion of the pros and cons of the various modes of derivatization, a comparison of derivatizations in CE with derivatizations in LC, the principles of fluorescence and prerequisites for a good fluorophore and the potent...Continue Reading

References

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Citations

Feb 13, 2001·Analytical Biochemistry·K L EwaltM Krihak
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·A BouchereauF Larher
Oct 4, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jana LangeChristine Wittmann
Oct 4, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qianfeng WengWenrui Jin
Jul 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ana M García-CampañaFermín Alés Barrero
Jul 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Frank WelderChrista L Colyer
Dec 15, 2004·Electrophoresis·Feliciano Priego-Capote, Maria D Luque de Castro
Dec 1, 2006·Electrophoresis·Ana M García-CampañaHuguette Fabre
Jun 2, 2007·Electrophoresis·Martin Pumera
Nov 28, 2007·Electrophoresis·Jie ZhangAnn Van Schepdael
Jul 2, 2014·Electrophoresis·Artem A Zdanov, Olga V Shuvaeva
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