PMID: 9541240Apr 16, 1998Paper

Derived protein and cDNA sequences of hamster amelogenin

European Journal of Oral Sciences
D M LyaruuJ P Simmer

Abstract

Hamster enamel protein extracts were analyzed by RP-HPLC and the isolated fractions by SDS-and Western blotting using polyclonal antibodies against recombinant mouse amelogenin and anti-peptide antibodies against the mouse exon 4-encoded sequence. Total RNA was extracted from enamel organ epithelia and, using a 3' rapid amplification of cDNA ends (3' RACE) technique, the coding regions for three different amelogenin isoforms were cloned along with the 3' non-coding region. DNA sequencing revealed that the hamster amelogenin isoforms are 180, 73 and 59 amino acids in length, respectively. The 59-residue amelogenin corresponds to the leucine-rich amelogenin protein (LRAP), the 73-residue amelogenin corresponds to LRAP with the inclusion of the exon 4-encoded sequence, while the 180-residue amelogenin is the most abundant amelogenin isoform. Edman degradation was performed on purified hamster amelogenin, which provided the amino acid sequence in the region encoded by the 5' PCR amplification primer used in cloning. Therefore, the entire derived amino acid sequence of hamster amelogenin was revealed. The hamster amelogenin amino acid sequence was aligned with all its known homologues. Hamster differs from rat and mouse amelogenin a...Continue Reading

Citations

Dec 20, 2002·Connective Tissue Research·James P Simmer, Jan C C Hu
Aug 12, 1999·Journal of Structural Biology·A G FinchamJ P Simmer
Nov 24, 2005·Journal of Dental Research·G StephanopoulosK Lyroudia
Mar 1, 2006·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xinping WangThomas G H Diekwisch
Mar 4, 2005·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Xinping WangThomas G H Diekwisch
May 6, 2006·European Journal of Oral Sciences·T G H DiekwischX Luan

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