Deriving Extracellular Vesicle Size From Scatter Intensities Measured by Flow Cytometry

Current Protocols in Cytometry
Leonie de RondEdwin van der Pol

Abstract

Flow cytometry is commonly used to investigate the potential for extracellular vesicles (EVs) to be biomarkers of disease. A typical flow cytometer detects fluorescence and scatter intensities of single EVs in arbitrary units. These arbitrary units complicate data interpretation and data comparison between different flow cytometers. For example, comparison of detected EV concentrations requires knowledge of the detectable EV sizes. Using Mie theory and knowledge of the optical configuration of the flow cytometer, EV size can be derived from the scatter intensity for a given EV refractive index. Here, a protocol is described to derive the size of EVs and other nanoparticles from the scatter intensity. The resulting size distribution allows the comparison of data between flow cytometers, which is a prerequisite for clinical application of EVs as biomarkers and may advance other fields where sizing of nanoparticles is essential. © 2018 by John Wiley & Sons, Inc.

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Citations

Feb 23, 2020·Viruses·Irene González-DomínguezFrancesc Gòdia
Jun 2, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Joshua A WelshVera A Tang
Sep 12, 2019·Scientific Reports·Laura SantucciGiovanni Candiano
Sep 17, 2020·Current Protocols in Cytometry·Joshua A Welsh, Jennifer C Jones
Sep 7, 2019·Journal of Extracellular Vesicles·L de RondF A W Coumans
Apr 27, 2020·Journal of Endocrinological Investigation·M FranzagoE Vitacolonna
Feb 6, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Leonie de RondFrank A W Coumans
Mar 5, 2020·Journal of Extracellular Vesicles·Joshua A WelshJennifer C Jones
Aug 31, 2020·Research and Practice in Thrombosis and Haemostasis·Linda G RikkertFrank A W Coumans
Nov 26, 2019·Journal of Extracellular Vesicles·René J BerckmansRienk Nieuwland
Mar 25, 2020·International Journal of Molecular Sciences·Danilo BucaPaola Lanuti
Jun 26, 2020·Frontiers in Oncology·L G RikkertL W M M Terstappen
Sep 24, 2020·American Journal of Reproductive Immunology : AJRI·Martine KuiperEdwin van der Pol
Oct 22, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Leonie de RondEdwin van der Pol
Nov 18, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Joshua A WelshAndré Görgens
Dec 31, 2020·International Journal of Molecular Sciences·Marco MarchisioPaola Lanuti
Oct 30, 2020·International Journal of Molecular Sciences·Pasquale SimeonePaola Lanuti
May 1, 2021·International Journal of Molecular Sciences·Sobha Karuthedom GeorgeViktoria Weber
Aug 14, 2021·Environmental Pollution·Carmen SorasanRoberto Rosal

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