Derlin1 functions as an oncogene in cervical cancer via AKT/mTOR signaling pathway

Biological Research
Ling LiPing Wei

Abstract

Cervical cancer (CC) ranks third in the morbidity and mortality of female cancer around the world. Derlin1 has been found to be overexpressed in several human cancers. However, it is still unclear about its roles in CC. The research aims to explore the relationship between Derlin1 and CC. We purchased a human CC tissues microarray, which contained CC tissues and corresponding para-cancerous tissues from 93 patients with primary cervical squamous cell carcinoma. Immunohistochemical staining was used to confirm the expression of Derlin1 in these tissues. And we detected the differential expression of Derlin1 in cervical cancer cell lines and normal cervical epithelial cells (H8). Further, the cervical cancer cell lines SiHa and C33A were used as an in vitro model, which was down-regulated the expression of Derlin1 using siRNA interference technology. The effects of Derlin1 down-regulating in CC cell lines on cell proliferation and migration were detected by CCK8 assay and transwell assay, respectively. The effect of Derlin1 down-regulating on apoptosis was analyzed by flow cytometry, and apoptosis-related proteins were detected using western blotting. In-depth mechanisms were studied using western blotting. In addition, the effec...Continue Reading

References

Jul 5, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·G MukherjeeM A Stanley
Nov 25, 2003·Oncogene·David G BreckenridgeGordon C Shore
Jun 16, 2004·Cancer Metastasis Reviews·Quynh-Thu LeAmato J Giaccia
Dec 18, 2004·Mutation Research·Martin Schröder, Randal J Kaufman
Oct 13, 2006·Nature·Mariusz KarbowskiRichard J Youle
Jul 22, 2008·International Journal of Radiation Oncology, Biology, Physics·Takamitsu HaraTomio Inoue
Mar 4, 2009·The Journal of Cell Biology·Sarah B BermanJ Marie Hardwick
Oct 6, 2010·Cancer·Petra BiewengaMarrije R Buist
Feb 2, 2011·Journal of the National Cancer Institute·Mark SchiffmanPhilip E Castle
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Oct 15, 2013·Pathobiology of Aging & Age Related Diseases·Paul Hasty, Barbara A Christy
Feb 5, 2014·Journal of Cancer·Patrizia ViciAldo Venuti

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Citations

Sep 8, 2020·The FEBS Journal·Colin Adrain, Miguel Cavadas

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Methods Mentioned

BETA
transfection
protein assay
flow cytometry

Software Mentioned

QUANTITY ONE
SPSS
Flowjo

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