Dermal fibroblast-associated gene induction by asiaticoside shown in vitro by DNA microarray analysis

The British Journal of Dermatology
L LuY Mao

Abstract

Asiaticoside, isolated from Centella asiatica, promotes fibroblast proliferation and extracellular matrix (ECM) synthesis in wound healing. The precise mechanism, however, in molecular and gene expression levels is still unclear. Using cDNA microarray technology, the alteration of gene expression profiles was determined for human dermal fibroblasts in vitro in the presence of asiaticoside (30 microg mL(-1)). Fifty-four genes, with known functions for cell proliferation, cell cycle progression and synthesis of ECM, were significantly upregulated in our 'genome-nest' expression profile at various time points. Furthermore, the mRNA levels and protein production of certain genes responsible for ECM synthesis (e.g. encoding type I and type III collagen proteins) were evaluated by Northern blot and radioimmunoassay, respectively. We found that there is a close correlation between the gene profile, mRNA and protein production in the response of the cells to asiaticoside stimulation. This information could be used for exploring the response of the target genes to asiaticoside in fibroblasts.

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Citations

Jul 2, 2010·Nutrition Reviews·Angela C B FerreiraLydia M Ferreira
Dec 17, 2009·Journal of Microencapsulation·Somsak SaesooUracha Rungsardthong Ruktanonchai
Dec 10, 2015·Journal of Cellular and Molecular Medicine·Ye ZhangTong Wang
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May 26, 2012·Phytotherapy Research : PTR·Nunthawan NowwarotePrasit Pavasant
Jan 9, 2014·Phytotherapy Research : PTR·Wiesława BylkaMałgorzata Brzezińska
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Feb 26, 2015·Journal of Tissue Engineering and Regenerative Medicine·Chun-Hsu YaoChiung-Hua Huang
Aug 16, 2019·BMC Complementary and Alternative Medicine·Afnan Sh AhmedZainul Amiruddin Zakaria

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