Dermal stem cells can differentiate down an endothelial lineage.

Stem Cells and Development
Emma BellNicholas Hole

Abstract

In this study, we have demonstrated that cells of neural crest origin located in the dermal papilla (DP) exhibit endothelial marker expression and a functional activity. When grown in endothelial growth media, DP primary cultures upregulate expression of vascular endothelial growth factor receptor 1 (FLT1) mRNA and downregulate expression of the dermal stem cell marker α-smooth muscle actin. DP cells have demonstrated functional characteristics of endothelial cells, including the ability to form capillary-like structures on Matrigel, increase uptake of low-density lipoprotein and upregulate ICAM1 (CD54) in response to tumour necrosis factor alpha (TNF-α) stimulation. We confirmed that these observations were not due to contaminating endothelial cells, by using DP clones. We have also used the WNT1cre/ROSA26R and WNT1cre/YFP lineage-tracing mouse models to identify a population of neural crest-derived cells in DP cultures that express the endothelial marker PECAM (CD31); these cells also form capillary-like structures on Matrigel. Importantly, cells of neural crest origin that express markers of endothelial and mesenchymal lineages exist within the dermal sheath of the vibrissae follicle.

References

Mar 1, 1988·The Journal of Investigative Dermatology·K S StennS J Tirrell
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G P NolanL A Herzenberg
Oct 1, 1993·The Journal of Investigative Dermatology·C A JahodaR F Oliver
Aug 12, 1999·The New England Journal of Medicine·R Paus, G Cotsarelis
Feb 22, 2001·The Journal of Clinical Investigation·K YanoM Detmar
Apr 12, 2001·BMC Developmental Biology·S SrinivasF Costantini
Sep 5, 2001·Nature Cell Biology·J G TomaF D Miller
Sep 24, 2002·Journal of Cell Science·Majlinda LakoColin A B Jahoda
Feb 26, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ronald WolfUlrike Blume-Peytavi
Mar 21, 2003·Developmental Biology·Viktor SzederMaya Sieber-Blum
Sep 25, 2003·Cell·Antonio P BeltramiPiero Anversa
Jan 13, 2004·Experimental Dermatology·Colin A B JahodaNicholas Hole
May 22, 2004·Stem Cells·Joachim OswaldCarsten Werner
Aug 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yasuyuki AmohRobert M Hoffman
Sep 15, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M Sieber-BlumV Szeder
Nov 2, 2004·Nature Cell Biology·Karl J L FernandesFreda D Miller
Feb 3, 2005·Stem Cells and Development·Mirela AnghelinaNicanor I Moldovan
Apr 28, 2005·The Journal of Investigative Dermatology·Gavin D RichardsonColin A B Jahoda
Nov 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yasuyuki AmohRobert M Hoffman
Jul 1, 2006·Science·Horace RheeElaine Fuchs
May 8, 2007·Seminars in Cell & Developmental Biology·James M WatersColin A B Jahoda
Mar 5, 2008·Cardiovascular Research·Jin Yu LiuStelios T Andreadis
Jul 12, 2008·Mechanisms of Development·Toshiyuki YoshidaSachiko Iseki
Nov 10, 2009·Current Opinion in Biotechnology·David P J HuntSiddharthan Chandran
Mar 13, 2010·Journal of Cellular Biochemistry·Ryoichi AkiRobert M Hoffman
Mar 27, 2010·Experimental Cell Research·Viljar JaksRune Toftgård
Nov 30, 2010·Cell Stem Cell·Nick BarkerHans Clevers

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Citations

Mar 13, 2014·Biological Reviews of the Cambridge Philosophical Society·Joseph N Nissimov, Asit Baran Das Chaudhuri
Apr 5, 2018·Stem Cell Research & Therapy·Aleksandra V MeleshinaElena V Zagaynova

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Methods Mentioned

BETA
PCR
flow cytometry
fluorescence-activated cell-sorting
FACS
transgenic

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