Dermaseptin, a peptide antibiotic, stimulates microbicidal activities of polymorphonuclear leukocytes

Biochemical and Biophysical Research Communications
B AmmarM Roch-Arveiller

Abstract

Dermaseptin (DRs S1), a 34-amino acid residue cationic antimicrobial peptide was studied for its effects on the production of reactive oxygen species (respiratory burst) and exocytosis of polymorphonuclear leukocytes (PMN). Treatment of PMN with DRs S1 (10-100 nM) stimulated significant production of reactive oxygen species (approximately a 2-fold increase relative to control) and release of myeloperoxidase. In addition, low DRs S1 concentrations (1-10 nM) primed the stimulation of respiratory burst induced by zymosan particles. In contrast to the native peptide, a dermaseptin fragment without either the COOH-terminal (DRs 1-10) or NH2 terminal (DRs 16-34) portion was inactive. The DRs S1-induced respiratory burst was inhibited by a selective protein kinase C inhibitor, GF 109203X, and was associated with early signalling events such as a rapid and transient elevation of cytosolic-free calcium concentration and phospholipase D activity. These data provide the first evidence of stimulating and priming properties of a peptide antibiotic on microbicidal activities of neutrophils, suggesting a potential role of dermaseptin in modulating host-defense mechanisms.

References

Mar 23, 1978·The New England Journal of Medicine·B M Babior
Jun 15, 1989·Biochemical and Biophysical Research Communications·A PerianinB Malfroy
Oct 1, 1985·The Journal of Clinical Investigation·T GanzR I Lehrer
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·B AgerberthG H Gudmundsson
Jan 1, 1993·Annual Review of Immunology·R I LehrerT Ganz
Jan 1, 1995·Annual Review of Microbiology·P Nicolas, A Mor

❮ Previous
Next ❯

Citations

Feb 19, 2002·Antimicrobial Agents and Chemotherapy·Shiri Navon-VeneziaAmram Mor
Feb 27, 2003·Antimicrobial Agents and Chemotherapy·Hongxia Zhao, Paavo K J Kinnunen
May 27, 2005·Antimicrobial Agents and Chemotherapy·Inna S RadzishevskyAmram Mor
Sep 29, 2001·The Journal of Peptide Research : Official Journal of the American Peptide Society·A C RinaldiM Simmaco
Jul 19, 2008·The FEBS Journal·Constance AuvynetYvonne Rosenstein
Mar 4, 2006·Molecular Pharmacology·Stéphanie PochetJean-Paul Dehaye
Jan 13, 2006·The Journal of Biological Chemistry·Deborah E ShalevAmram Mor
Feb 27, 1999·Current Opinion in Immunology·R I Lehrer, T Ganz
Dec 22, 1999·Current Opinion in Chemical Biology·D G McCaffertyR Kruger
Jul 30, 1999·Biochimica Et Biophysica Acta·L C McPhailS Sergeant
Oct 25, 2003·Biochemical and Biophysical Research Communications·Leentje MoermanSuzanne Bosteels
Oct 22, 2008·Biochimica Et Biophysica Acta·Pierre Nicolas, Chahrazade El Amri
Dec 3, 2008·Biochimica Et Biophysica Acta·Luis RivasDavid Andreu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.