Dermaseptin-S1 decreases Candida albicans growth, biofilm formation and the expression of hyphal wall protein 1 and aspartic protease genes

Journal of Applied Microbiology
A BelmadaniMahmoud Rouabhia

Abstract

This study aimed to investigate the effect of synthetic antimicrobial peptide dermaseptin-S1 (DS1) (ALWKTMLKKLGTMALHAGKAALGAADTISQGTQ) on the growth of Candida albicans, its transition from blastospore to hyphae, and its biofilm formation. We also analysed the expression of different genes (HWP1 and SAPs) involved in C. albicans virulence. Using cell count we showed that in addition to decreasing C. albicans growth, peptide DS1 inhibited its transition from blastospore to hyphal form. These effects are comparable to those obtained with amphotericin B (AmB). Electron microscopy analyses showed that C. albicans cells treated with either DS1 or AmB displayed a distorted cell wall surface, suggesting that the effect of DS1 was similar to that of AmB on C. albicans cell membrane structure. These observations were confirmed by our results with biofilms showing that both DS1 peptide and AmB significantly inhibited biofilm formation after 2 and 4 days. The effect of DS1 on C. albicans growth, transition and biofilm formation may occur through gene modulation, as the expression of HWP1, SAP1, SAP2, SAP3, SAP9 and SAP10 genes involved in C. albicans pathogenesis were all downregulated when C. albicans was treated with DS1. DS1 inhibits t...Continue Reading

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Citations

Jan 24, 2019·International Journal of Environmental Research and Public Health·Humidah AlanaziMahmoud Rouabhia
Dec 12, 2018·Frontiers in Microbiology·Subramanian MuthamilShunmugiah Karutha Pandian
Jul 22, 2020·Probiotics and Antimicrobial Proteins·Johan Samot, Mahmoud Rouabhia
Aug 24, 2019·Protein and Peptide Letters·Jaspreet Kaur Boparai, Pushpender Kumar Sharma
Nov 5, 2020·MBio·Giuseppe Buda De CesareMichael C Lorenz

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