PMID: 19916908Nov 18, 2009Paper

Dermatopontin is expressed in human liver and is downregulated in hepatocellular carcinoma

Biochemistry. Biokhimii︠a︡
Xirong LiChongjie Zhang

Abstract

Dermatopontin (DPT) was recently found as a downstream target of vitamin D receptor, which is a key molecule in the 1,25-dihydroxy-vitamin D(3) anti-hepatoma proliferation pathway. MCTx-1 from Millepora, a homolog of DPT, is identified as a cytotoxin towards leukemia cells. The aim of this study was to analyze DPT expression in hepatocellular carcinoma (HCC) based on the analysis for DPT gene in normal tissues in order to estimate its function in the progression of HCC. DPT mRNA expression was analyzed in normal tissues and HCC cell lines by RT-PCR, and in HCC tissue by RT-PCR and real-time PCR. Its protein was examined in HCC tissues by Western blot and immunohistochemistry assays. Meanwhile, transforming growth factor-beta1 (TGF-beta1) that is closely associated with HCC and DPT was observed by immunohistochemistry in HCC tissues. The results showed that DPT mRNA was strongly expressed in human fetal and adult liver, kidney, and spleen, weakly in ovary and heart, and absent in other tissues and HCC cell lines examined. Its mRNA was significantly downregulated in HCC tissues, while its protein was weakly expressed in tumor compared with non-tumor. DPT is located mainly in the cytoplasm of several cell types in the liver; it ha...Continue Reading

References

Jul 11, 2002·The American Journal of Pathology·Gianluigi GiannelliSalvatore Antonaci
Sep 17, 2002·The Journal of Investigative Dermatology·Ushio TakedaHiroshi Shinkai
Apr 30, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marielle Gascon-BarréAntonio Nanci
Oct 31, 2003·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Do Youn ParkChul Hoon Kim
May 13, 2004·Genes, Chromosomes & Cancer·William H CatherinoJames H Segars
May 27, 2005·Seminars in Liver Disease·Morris Sherman
Sep 22, 2006·Connective Tissue Research·Osamu Okamoto, Sakuhei Fujiwara
Jun 6, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Radhika R PochampallyDarwin J Prockop
Nov 7, 2007·Biochemical and Biophysical Research Communications·Akiko IguchiHiroshi Nagai
Mar 6, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Emilia FransveaGianluigi Giannelli

❮ Previous
Next ❯

Citations

Dec 25, 2012·Matrix Biology : Journal of the International Society for Matrix Biology·Xiaoyan LiuYingjie Wei
Oct 3, 2012·Experimental and Molecular Pathology·Timothy M StearnsAnnerose Berndt
Dec 10, 2014·Scientific Reports·Venkat Raghavan Krishnaswamy, Purna Sai Korrapati
Aug 17, 2014·Radiation Oncology·Mariola Fotin-MleczekStephan M Huber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Related Papers

International Journal of Cancer. Journal International Du Cancer
Masanobu YamatojiKatsuhiro Uzawa
Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Ying-Jie WeiSheng-Shou Hu
© 2022 Meta ULC. All rights reserved