Dermatopontin promotes epidermal keratinocyte adhesion via alpha3beta1 integrin and a proteoglycan receptor

Biochemistry
Osamu OkamotoSakuhei Fujiwara

Abstract

Dermatopontin, an extracellular matrix component initially purified from bovine dermis, promoted cell adhesion of the human epidermal keratinocyte cell line (HaCaT cells). HaCaT cells spread on dermatopontin and formed actin fibers. Adhesion of HaCaT cells to dermatopontin was inhibited by both EDTA and heparin and was mediated in part by alpha3beta1 integrin. A synthetic peptide (DP-4, PHGQVVVAVRS; bovine dermatopontin residues 33-43) specifically inhibited adhesion of cells to dermatopontin, and when the DP-4 peptide was coated on the well, it promoted cell adhesion in a dose-dependent manner. An active core sequence of the DP-4 peptide was localized to an eight-amino acid sequence (GQVVVAVR). These results indicate that dermatopontin is a novel epidermal cell adhesion molecule and suggest that the DP-4 sequence is critical for the cell adhesive activity of dermatopontin. Adhesion of cells to DP-4 was strongly inhibited by heparin. When HaCaT cells were treated with heparitinase I, the cells failed to adhere to DP-4 but chondroitinase ABC treatment did not influence the adhesion activity. DP-4 specifically interacted with biotinylated heparin, and this interaction was inhibited by unlabeled heparin. DP-4 peptide significantly...Continue Reading

References

Aug 1, 1991·The Journal of Cell Biology·K EleniusM Jalkanen
Nov 14, 1997·The Biochemical Journal·D J Carey
Oct 29, 1998·The Journal of Biological Chemistry·J K LangfordR D Sanderson
Nov 26, 1998·The Journal of Biological Chemistry·M NomizuY Yamada
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SaoncellaP F Goetinck
Sep 2, 1999·The New England Journal of Medicine·A J Singer, R A Clark
Oct 27, 2001·Clinical and Experimental Dermatology·E A O'Toole
Jul 4, 2002·Fertility and Sterility·John C M TsibrisWilliam N Spellacy
Sep 17, 2002·The Journal of Investigative Dermatology·Ushio TakedaHiroshi Shinkai
Jan 2, 2003·The Journal of Biological Chemistry·Charles Kumar ThodetiUlla M Wewer
Apr 16, 2003·The Journal of Cell Biology·Zohreh Mostafavi-PourMartin J Humphries
Aug 30, 2003·The Journal of Biological Chemistry·Osamu OkamotoPatricia Rousselle
Dec 12, 2003·Drugs of Today·Anna T Grazul-BilskaKay M Abdullah
Feb 10, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Robert F Diegelmann, Melissa C Evans
May 13, 2004·Genes, Chromosomes & Cancer·William H CatherinoJames H Segars
Oct 14, 2004·The Journal of Cell Biology·DeannaLee M BeauvaisAlan C Rapraeger
Aug 11, 2006·The Journal of Endocrinology·Takumi TakeuchiTadaichi Kitamura
Aug 29, 2006·Matrix Biology : Journal of the International Society for Matrix Biology·Constance Y Fears, Anne Woods
Sep 22, 2006·Connective Tissue Research·Osamu Okamoto, Sakuhei Fujiwara
Nov 14, 2006·The International Journal of Biochemistry & Cell Biology·Annika N AlexopoulouJohn R Couchman
Feb 15, 2007·The Journal of Investigative Dermatology·Sabine A EmingJeffrey M Davidson
May 9, 2007·Frontiers in Bioscience : a Journal and Virtual Library· RajaR Rivkah Isseroff
Jun 21, 2007·Journal of Cellular Physiology·Sophie BachyPatricia Rousselle
Aug 8, 2007·Journal of Cellular Physiology·Allison L Berrier, Kenneth M Yamada
Nov 7, 2007·Biochemical and Biophysical Research Communications·Akiko IguchiHiroshi Nagai

❮ Previous
Next ❯

Citations

Sep 28, 2014·Cell and Tissue Research·Venkat Raghavan KrishnaswamyPurna Sai Korrapati
Dec 25, 2012·Matrix Biology : Journal of the International Society for Matrix Biology·Xiaoyan LiuYingjie Wei
Jul 24, 2013·The Journal of Investigative Dermatology·Weimin WuSakuhei Fujiwara
Jul 29, 2011·International Journal of Cancer. Journal International Du Cancer·Masanobu YamatojiKatsuhiro Uzawa
Aug 2, 2014·Journal of Dermatological Science·Weimin WuSakuhei Fujiwara
Aug 6, 2014·Journal of Dermatological Science·Aiko KatoSakuhei Fujiwara
Dec 10, 2014·Scientific Reports·Venkat Raghavan Krishnaswamy, Purna Sai Korrapati
Mar 15, 2011·The Journal of Biological Chemistry·Aiko KatoSakuhei Fujiwara
Oct 13, 2015·World Journal of Gastroenterology : WJG·Mitsuro KandaYasuhiro Kodera
Dec 1, 2020·PloS One·Trikkur Madom Seetaraman AmrithaAnuradha Dhanasekaran

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.