PMID: 9164997May 1, 1997Paper

Desaturation of stearate is insufficient to increase the concentrations of oleate in cultured rat hepatocytes

The Journal of Nutrition
T Pai, Y Y Yeh

Abstract

Desaturation of stearate and palmitate and its effect on cellular accumulation of oleate were determined in primary culture of rat hepatocytes. The rate of oleate synthesis as measured by the formation of monounsaturated fatty acids from stearate was significantly higher than that from palmitate. The rate of [1-(14)C]stearate incorporation into oleate [1208 +/- 195 pmol/(mg protein x 4 h)] was 80% higher than that of [1-(14)C]palmitate [(672 +/- 82 pmol/(mg protein x 4 h)]. Despite the different rates of desaturation, the cellular oleate concentrations did not differ in the cells treated with stearate and palmitate (i.e., 42.5 +/- 4.5 vs. 40.8 +/- 5.2 nmol/mg protein). On the other hand, oleate concentration in the cells incubated with exogenous oleate was 198.1 +/- 9.5 nmol/mg protein. There was a dose-dependent increase in cellular stearate concentration by increasing stearate concentrations from 0.5 mmol/L to 4.0 mmol/L in culture medium. A linear increase in cellular stearate concentration was also achieved by increasing the duration of incubation with 1.0 mmol/L stearate from 2 to 24 h. Despite the marked increases in stearate concentrations under these conditions, oleate concentrations remained unchanged in the cells. The...Continue Reading

References

Dec 1, 1991·The American Journal of Clinical Nutrition·M A Denke, S M Grundy
Jan 1, 1991·Annals of Nutrition & Metabolism·M Mancini, M Parillo
Feb 26, 1991·Biochimica Et Biophysica Acta·E N ChristiansenA C Rustan
Nov 1, 1989·The Journal of Nutrition·A Bonanome, S M Grundy
Nov 1, 1989·The American Journal of Clinical Nutrition·D C MitchellP M Kris-Etherton
May 12, 1988·The New England Journal of Medicine·A Bonanome, S M Grundy
May 1, 1988·The American Journal of Clinical Nutrition·S M Grundy, G L Vega
Dec 1, 1986·American Journal of Epidemiology·A KeysM H Keys
Nov 1, 1965·The American Journal of Clinical Nutrition·D M HegstedF J Stare
Oct 4, 1965·Biochimica Et Biophysica Acta·J Elovson
Jan 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·I L KlingenbergS B Smith
May 11, 1957·Lancet·E H AHRENSM L PETERSON
Jul 1, 1965·Metabolism: Clinical and Experimental·Ancel KeysFrancisco Grande

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