Descemet's membrane as membranous support in RPE/IPE transplantation

Current Eye Research
G ThumannK Heimann

Abstract

The correct orientation of retinal pigment epithelium (RPE) cells is necessary for the integrity and proper function of the retina. For transplantation of RPE/iris pigment epithelium (IPE) grafts to the subretinal space in age-related macular degeneration, this cellular orientation is most effectively provided by a membranous support. The goal of this study was to establish an autologous or homologous membrane as a substratum for the growth of RPE/IPE. Porcine and bovine RPE and IPE were placed in primary culture on a dissected sheet (5 x 5 mm) of autologous porcine and bovine Descemet's membrane in slide chambers and grown to confluence. RPE and IPE cells cultured on Descemet's membrane form an intact monolayer. Light and electron microscopy showed the formation of both an intact monolayer and microvilli in both cell types. Since the slow host-graft rejection appears to play an important role in the failure of RPE transplantation in the subretinal space, it is critical to be able to transplant autologous materials. The techniques presented here establish a novel means to culture RPE or IPE cells on autologous Descemet's membrane where they form a "cell monolayer patch," consisting of a fragment of Descemet's membrane with cult...Continue Reading

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Citations

Nov 5, 2011·Regenerative Medicine·Sharon L MasonCarl M Sheridan
Mar 15, 2006·Current Eye Research·Gabriele ThumannPeter Wiedemann
Jul 10, 2012·Progress in Retinal and Eye Research·Magdalene J Seiler, Robert B Aramant
Feb 16, 2013·Ophthalmic Surgery, Lasers & Imaging Retina·Theodore Leng, Harvey A Fishman
Oct 9, 2007·Progress in Retinal and Eye Research·Lyndon da CruzPete Coffey
May 29, 2007·Progress in Retinal and Eye Research·Susanne BinderCarl Glittenberg
Jun 5, 2003·Acta Ophthalmologica Scandinavica·Carmen CapeánsManuel Sánchez-Salorio
Apr 25, 2012·Journal of Biomedical Materials Research. Part a·Andrew J TreharneAndrew J Lotery
Jun 27, 2015·Progress in Retinal and Eye Research·Hossein NazariMark S Humayun
Oct 27, 2014·Cell Biochemistry and Biophysics·Xiaobing WangGuomin Zhou
Sep 15, 2018·Journal of Biomaterials Applications·Sahar BalagholiNarsis Daftarian
Mar 8, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Julia BeutelSalvatore Grisanti
Feb 20, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sara Royce Hynes, Erin B Lavik
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Jun 8, 2001·Progress in Retinal and Eye Research·R D LundJ M Lawrence
Dec 12, 2003·Experimental Eye Research·Hao WangMarco A Zarbin

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