Description and phylogenetic analyses of ribosomal transcription units from species of Fasciolidae (Platyhelminthes: Digenea)

Journal of Helminthology
T H LeS P Lawton

Abstract

Many members of Fasciolidae are common trematodes in cattle, buffaloes, sheep, elephants, pigs, with some capable of infecting humans also. In this study, the complete or near-complete sequences of ribosomal transcription unit (rTU or rDNA), each of Fasciola hepatica (Australia), Fascioloides jacksoni (Sri Lanka), Fasciolopsis buski (Vietnam) and three isolates of F. gigantica (Vietnam), were obtained and characterized. The full length of rDNA for each F. hepatica, 'hybrid' Fasciola sp., Fas. jacksoni and Fa. Buski, was 7657 bp, 7966 bp, 7781 bp and 8361 bp, with the complete intergenic spacer region (IGS) (862 bp, 1170 bp, 987 bp and 561 bp), respectively. The rDNA of two 'pure' F. gigantica isolates from Vietnam was 6794 bp with unsequenced IGS. For 28S rRNA genes the Fasciola spp. are equal, 1958 bp for 18S, 160 bp for 5.8S, 3863 bp and 454 bp for ITS1 but ITS2 differ by one nucleotide (Thymine) (359 or 360 bp). The ITS1 of the sensu lato Fa. buski has some distinguishable features, 286 bp for ITS2, 3862 bp for 28S and four repeat units of 356-361 bp each found in ITS1. The 28S rDNA analysis showed the lowest level of divergence (0-0.57%) between F. hepatica and F. gigantica and higher (2.23-2.62%) and highest (6-6.42%) for ...Continue Reading

References

Dec 24, 1998·Nucleic Acids Research·G Benson
Jun 20, 2003·International Journal for Parasitology·P D OlsonD T J Littlewood
Mar 18, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·Thanh Hoa LeDonald P McManus
Nov 17, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·M V PeriagoS Mas-Coma
Jan 29, 2010·Parasitology Research·Madoka Ichikawa, Tadashi Itagaki
Feb 1, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Guang-Hui ZhaoXing-Quan Zhu
Aug 21, 2014·TheScientificWorldJournal·Xu ZhengXing-Quan Zhu
Dec 25, 2015·International Journal for Parasitology·Vasyl V TkachAneta Kostadinova
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Thanh Hoa LeDavid Blair
Jul 13, 2019·Advances in Experimental Medicine and Biology·Santiago Mas-ComaMª Dolores Bargues
Aug 27, 2019·Trends in Genetics : TIG·Amanda V Cerqueira, Bernardo Lemos

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