Description of Echinococcus granulosus genotypes in human hydatidosis in a region of southern Chile

Parasitology International
Carlos ManterolaJuan Carlos Roa

Abstract

Echinococcus granulosus species has a wide variety in both geography and hosts; indeed, 10 genotypes have been reported in studies on material of animal origin. The aim of this study was to genotype E. granulosus obtained from human hydatid cysts. The hydatid fluid and sand was collected from patients who underwent surgery for hepatic and pulmonary hydatidosis at Hospital Regional in Temuco, Chile, between 2004 and 2005. Two PCR systems were used: PCR Eg 9 and PCR Eg 16. The RsaI enzyme was used for RFLP. The genotype was confirmed using the sequence of one fragment of 366 bp from a mitochondrial gene (cox1). The DNA of protoscolices from 24 samples was analyzed, 4 of them from pulmonary cysts and 20 from hepatic cysts. The 366 bp fragment was amplified in 20 out of 24 samples (83.3%). Enzymatic digestion revealed the presence of 3 possible genotypes: in 20 out of 21 samples (95,2%), a restriction was observed corresponding to the G1 or G7 genotypes; in the remaining sample genotype G4 or G7 was observed. Sequencing confirmed the presence of G1 genotype for 19 samples and G6 genotype for the remaining sample (G4 or G7 according to PCR-RFLP). The PCR-RFLP technique enabled three possible genotypes present (G1 or G7, G4 or G7) to...Continue Reading

References

Nov 1, 1993·International Journal for Parasitology·J Bowles, D P McManus
May 25, 1994·Nucleic Acids Research·M R MicheliE D'Ambrosio
Jun 11, 1999·Parasitology·M C RosenzvitD P McManus
Oct 16, 2002·Trends in Parasitology·R C Andrew Thompson, Donald P McManus
Mar 5, 2003·Experimental Parasitology·L M GonzálezC Cuesta-Bandera
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Laura KamenetzkyMara C Rosenzvit
Mar 27, 2004·Parasitology·D P McManus, R C A Thompson
Jul 6, 2004·Acta Tropica·K Daniel MwambeteC Cuesta-Bandera

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Citations

Sep 30, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Adriano CasulliCarlo Filice
Jan 1, 2010·Mediterranean Journal of Hematology and Infectious Diseases·Abdulkadir KüçükbayrakZeynep Seçkin Küçükbayrak
Jun 26, 2014·Veterinary Parasitology·Silke RiesleRodolfo Paredes
Nov 27, 2015·Tropical Medicine & International Health : TM & IH·Marcela Alejandra CucherLaura Kamenetzky
Dec 21, 2011·Asian Pacific Journal of Tropical Medicine·Gholamreza MowlaviAbdoreza Salahi-Moghaddam
Mar 28, 2012·Asian Pacific Journal of Tropical Medicine·Shyamapada Mandal, Manisha Deb Mandal
Nov 26, 2013·International Journal for Parasitology·Cristian A Alvarez RojasMarshall W Lightowlers
Oct 23, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Teivi LaurimäeUrmas Saarma
Dec 24, 2014·The American Journal of Tropical Medicine and Hygiene·Sima RostamiMajid Fasihi Harandi
Jul 25, 2019·Tropical Animal Health and Production·Fadya Abdullah AlKitaniDerek Roberts
Sep 15, 2016·Parasites & Vectors·Cristian A Alvarez RojasUNKNOWN Ministry of Health, SAG and other organizations attending the meeting
Jul 16, 2021·Acta Parasitologica·Carlos ManterolaNayely García-Méndez

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