Description of Sarcocystis scandentiborneensis sp. nov. from treeshrews (Tupaia minor , T. tana ) in northern Borneo with annotations on the utility of COI and 18S rDNA sequences for species delineation

International Journal for Parasitology. Parasites and Wildlife
Paula Ortega PérezThomas Jäkel

Abstract

Sarcocystis scandentiborneensis sp. nov. was discovered in histological sections of striated musculature of treeshrews (Tupaia minor, T. tana) from Northern Borneo. Sarcocysts were cigar-shaped, 102 μm-545 μm long, and on average 53 μm in diameter. The striated cyst wall varied in thickness (2-10 μm), depending on whether the finger-like, villous protrusions (VP) were bent. Ultrastructurally, sarcocysts were similar to wall type 12 but basal microtubules extended into VPs that tapered off with a unique U-shaped, electron-dense apical structure. In phylogenetic trees of the nuclear 18S rRNA gene, S. scandentiborneensis formed a distinct branch within a monophyletic subclade of Sarcocystis spp. with (colubrid) snake-rodent life cycle. We mapped all intraspecific (two haplotypes) and interspecific nucleotide substitutions to the secondary structure of the 18S rRNA gene: in both cases, the highest variability occurred within helices V2 and V4 but intraspecific variability mostly related to transitions, while transition/transversion ratios between S. scandentiborneensis, S. zuoi, and S. clethrionomyelaphis were skewed towards transversions. Lack of relevant sequences restricted phylogenetic analysis of the mitochondrial Cytochrome C...Continue Reading

References

Dec 10, 1997·Journal of Wildlife Diseases·T JäkelS Hongnark
Oct 14, 2000·Parasite : Journal De La Société Française De Parasitologie·D ModrýJ R Slapeta
Sep 28, 2002·International Journal for Parasitology·Odile Mercereau-PuijalonEmmanuel Bischoff
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Nov 1, 1960·Stain Technology·K C RICHARDSONE H FINKE
Mar 12, 2004·International Journal for Parasitology·David A MorrisonJens G Mattsson
Dec 14, 2004·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Sudhindra R GadagkarSudhir Kumar
Nov 16, 2005·Annual Review of Genetics·Masatoshi Nei, Alejandro P Rooney
Dec 1, 2006·Advances in Parasitology·David A Morrison
Apr 29, 2008·Nucleic Acids Research·Mark JohnsonThomas L Madden
May 17, 2008·Nucleic Acids Research·Sébastien MorettiCédric Notredame
Jul 29, 2009·Trends in Parasitology·David A Morrison
May 14, 2011·Molecular Ecology Resources·UNKNOWN Molecular Ecology Resources Primer Development ConsortiumLvping Zhang
Dec 14, 2011·The Journal of Parasitology·Jun-Jie HuJing-Ling Song
Feb 24, 2012·Systematic Biology·Fredrik RonquistJohn P Huelsenbeck
Jul 31, 2012·Free Radical Biology & Medicine·Satish Srinivasan, Narayan G Avadhani
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jan 27, 2015·Current Biology : CB·Matthew J StruebigStephanie Kramer-Schadt
Mar 6, 2015·Molecular Biology and Evolution·S Blair HedgesSudhir Kumar
Mar 17, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Brianna L S StengerJohn M McEvoy
Jun 6, 2015·Parasitology Research·Niichiro AbeKazutoshi Takami
Nov 22, 2015·Nucleic Acids Research·Karen ClarkEric W Sayers
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Oct 14, 2016·Scientific Reports·Mikko PentinsaariTomas Roslin
May 21, 2017·BMC Genomics·Cong GuoTimothy E Reddy
Nov 18, 2017·Genome Biology and Evolution·David O F SkibinskiJonathan G L Mullins

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Datasets Mentioned

BETA
KF309701
FJ827485-88
MN732561
MN733816
KU341120

Methods Mentioned

BETA
transmission electron microscopy
light microscopy
Electrophoresis
electron microscopy
PCR

Software Mentioned

BLAST
MUSCLE
Coffee
FigTree
MEGA
MrBayes
R

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