DOI: 10.1101/496661Dec 17, 2018Paper

Description of Schaedlerella arabinophila gen. nov., sp. nov., a D-arabinose utilizing bacterium isolated from feces of C57BL/6J mice and a close relative of Clostridium sp. ASF 502

BioRxiv : the Preprint Server for Biology
Melissa SohHenning Seedorf

Abstract

The use of gnotobiotics has gained large interest in recent years due to technological advances that have revealed the importance of host-associated microbiomes for host physiology and health. One of the oldest and most important gnotobiotics mouse model, the Altered Schaedler Flora (ASF) has been used for several decades. ASF comprises eight different bacterial species, which have been characterized to different extent, but only few are available through public strain collections. Here, the isolation of a close relative to one of the less studied ASF strains, Clostridium sp. ASF 502, is reported. Isolate TLL-A1, which shares 99.6% 16S rRNA gene sequence identity with Clostridium sp. ASF 502, was obtained from feces of C57BL/6J mice where is was detectable at a relative abundance of less than one percent. D-arabinose was used as sole carbon source in the anaerobic cultivation medium. Growth experiments with TLL-A1 on different carbon sources and analysis of its ~6.5 gigabase genome indicate that TLL-A1 harbors a large gene repertoire to utilize different carbohydrates for growth. Comparative genome analyses of TLL-A1 and Clostridium sp. ASF 502 reveal differences in genome content between the two strains, in particular with reg...Continue Reading

Related Concepts

Arabinose
Carbohydrates
Carbon
Genes
Genome
Physiological Aspects
Isolation Aspects
Research Study
Comparative Genomic Analysis
Analysis

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

International Journal of Systematic and Evolutionary Microbiology
Olga I NedashkovskayaKyung Sook Bae
International Journal of Systematic and Evolutionary Microbiology
Olga I NedashkovskayaKyung Sook Bae
International Journal of Systematic and Evolutionary Microbiology
Ho-Won ChangJin-Woo Bae
© 2021 Meta ULC. All rights reserved