PMID: 8947490Nov 15, 1996Paper

Desensitization of P2U receptor in neuronal cell line. Different control by the agonists ATP and UTP, as demonstrated by single-cell Ca2+ responses

The Biochemical Journal
U Czubayko, G Reiser

Abstract

The neuronal cell line NG108-15 (180CC15) responds to extracellular stimuli of ATP or UTP with a transient increase in the level of cytosolic Ca2+. Desensitization was investigated by recording single-cell Ca2+ responses induced by consecutive, regularly spaced (100 s intervals), brief pulses of the nucleotides. The two natural ligands of the P2U receptor, ATP and UTP, were applied at a concentration that evoked responses of a comparable size. With two pulses of UTP (10 microM), a substantial decrease (of 43%) was observed in the size of the second response. The magnitude of response was determined by measuring either the maximal amplitude or the total response, represented by the area of the Ca2+ transient. The analogous studies with ATP pulses showed a much smaller decrease (of 12%). Comparable experiments performed to investigate the mutual interaction between ATP and UTP revealed that after stimulation with ATP the response to UTP was slightly (12%) diminished, whereas the response to ATP after UTP was greatly (52%) decreased. The different degree of desensitization by either UTP or ATP of P2U receptors could be due to (1) a difference in the mode of activation of the receptor by the two ligands or (2) recruitment of anothe...Continue Reading

Citations

Feb 26, 2015·Journal of Biochemistry·Sayo YashimaHideyoshi Higashi
Feb 18, 2017·Biochemical and Biophysical Research Communications·Tetsuto NakagawaHideyoshi Higashi
May 15, 2007·Biomechanics and Modeling in Mechanobiology·Kai Rong QinZong Lai Jiang
Jan 16, 1999·Biochimica Et Biophysica Acta·S PedersenE K Hoffmann
Aug 6, 2005·Neurochemistry International·Katrin Sak, Peter Illes

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