PMID: 15252285Jul 15, 2004Paper

Desensitization of PKA-stimulated ciliary beat frequency in an ethanol-fed rat model of cigarette smoke exposure

Alcoholism, Clinical and Experimental Research
Todd A WyattJoseph H Sisson

Abstract

Our previous studies have shown that the ciliary beat frequency (CBF) of cultured ciliated airway epithelial cells exposed to chronic ethanol fails to increase in response to beta-agonist stimulation. This loss of the ciliary "flight response" correlates with an ethanol-mediated desensitization of adenosine 3':5'-cyclic monophosphate-dependent protein kinase (PKA), a known regulatory component of CBF stimulation. We hypothesized that a similar ethanol-mediated desensitization of CBF would occur in vivo. Sprague Dawley rats were fed a liquid diet containing various concentrations of ethanol for 1 or 5 weeks. Half were exposed to cigarette smoke for 12 weeks and half were sham exposed. Animals were killed and tracheal epithelial cells analyzed for CBF and PKA activity. Baseline CBF (approximately 6 Hz) was unchanged in tracheal epithelial cells of rats consuming diets containing 0-36% ethanol for 5 weeks. Isoproterenol stimulated CBF to 12 to 13 Hz in the tracheal epithelial cells of control rats not administered ethanol. However, isoproterenol stimulation of CBF was blunted to 7.5 Hz in rats eating a 26% ethanol diet, and there was no stimulation of CBF in rats fed a diet containing 36% ethanol. Similarly, isoproterenol stimulat...Continue Reading

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Citations

Jan 28, 2012·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Chelsea R NavarretteTodd A Wyatt
Dec 3, 2009·Tissue Engineering. Part C, Methods·Yuchi WangHua Mao
Nov 25, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Peter J OldenburgJoseph H Sisson
Oct 22, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·P J OldenburgJ H Sisson
Nov 3, 2006·American Journal of Respiratory Cell and Molecular Biology·Margaret K ElliottTodd A Wyatt
Mar 7, 2012·American Journal of Rhinology & Allergy·David GudisNoam A Cohen
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Feb 13, 2016·International Forum of Allergy & Rhinology·Do-Yeon ChoBradford A Woodworth
Oct 31, 2013·Alcohol·Samantha M SimetJoseph H Sisson
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Nov 21, 2009·Journal of Cardiothoracic and Vascular Anesthesia·Ankit MaheshwariDaniel I Sessler
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Sep 4, 2007·Alcohol·Joseph H Sisson
Apr 19, 2005·Alcoholism, Clinical and Experimental Research·John R SpurzemTodd A Wyatt
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Jan 13, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael E PriceTodd A Wyatt
Apr 15, 2018·Alcohol·Holly J HulsebusElizabeth J Kovacs

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