Desiccating stress decreases apical corneal epithelial cell size--modulation by the metalloproteinase inhibitor doxycycline.

Cornea
Robert M BeardsleyStephen C Pflugfelder

Abstract

We investigated the effects of desiccating stress on murine corneal apical epithelial cell area and desquamation by using 4 defined parameters and evaluated the effects of the metalloproteinase inhibitor doxycycline on this process. C57BL/6 mice were subjected to experimental dry eye (EDE) for 5 days without or with topical therapy with doxycycline 0.025% or 0.0025% or vehicle 4 times a day. C57BL/6 mice that were not exposed to desiccating stress served as controls. Whole mount corneas from each group were immunostained for occludin and visualized by laser scanning confocal microscopy. The images were analyzed in a masked fashion, and mean individual cell area, apical cell density, average cell number loss, and average percent loss were recorded. EDE caused a significant decrease in apical corneal cell area (1073 +/- 135.9 microm2), an increase in apical cell density (895.8 +/- 115.4 cells per mm2) and a greater percent of epithelial loss (21.29% +/- 13.40%) than controls (1341 +/- 95.28 microm2, 714.4 +/- 55.60 cells per mm2, 2.897% +/- 3.452%, P < 0.001 for all, respectively). Treatment with 0.025% doxycycline preserved cell area (1337 +/- 144.6 microm2) and the apical cell density (721.0 +/- 91.62 cells per mm2) and decreas...Continue Reading

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Citations

Jan 11, 2012·Archives of Ophthalmology·William StevensonReza Dana
Jun 27, 2012·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Gemma JulioMiquel Ralló
Jan 31, 2013·Investigative Ophthalmology & Visual Science·Po-Ting YehBen J Glasgow
Apr 19, 2016·Scientific Reports·Cintia S de PaivaStephen C Pflugfelder
Aug 17, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Thomas J Federici
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Jan 31, 2019·Mucosal Immunology·Fang BianCintia S de Paiva
May 30, 2020·PloS One·Stephanie J WanSuzanne M J Fleiszig
Jul 30, 2020·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Jehan AlamStephen C Pflugfelder
Feb 24, 2021·The Ocular Surface·Jeremias G Galletti, Cintia S de Paiva
Mar 5, 2021·Experimental Eye Research·Alireza ShoariMohammad Javad Rasaee
Oct 11, 2020·Experimental Eye Research·Stephen C Pflugfelder, Michael E Stern

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