Design a fluorometric aptasensor based on CoOOH nanosheets and carbon dots for simultaneous detection of lysozyme and adenosine triphosphate

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Zeinab SaberiAli Asghar Ensafi

Abstract

Simultaneous detection of biomarkers and biomolecules with great analytical performance still is challenging. A simple fluorometric dual-functional aptasensor was designed to detect Lysozyme (LYS) and adenosine triphosphate (ATP) as models of a protein and a small molecule simultaneously. The sensing principle of the aptasensor is based on the interactions between cobalt oxyhydroxide CoOOH nanosheets as fluorescence quencher and carbon dots (CDs) as fluorophores. The aptamer labeled with CDs was able to assemble on CoOOH nanosheets and consequently, the fluorescence signal was quenched. With addition target analytes to the system, the aptamers folded around of targets with a strong and specific affinity. Therefore, the labeled aptamer with CDs was detached from CoOOH nanosheets and the fluorescence signal was restored. The fluorescence spectral overlap of these two CDs is the main limitation for the simultaneous analysis. The least squared support vector machine (LS-SVM) was applied to resolve this problem. Under optimal conditions, when LS-SVR was used, detection limits were found 4.0 and 1.8 nmol L-1 for ATP and LYS. The parallel biosensor is capable of monitoring ATP and LYS levels in the biological samples with satisfactory...Continue Reading

Citations

Sep 3, 2020·Analytical and Bioanalytical Chemistry·Mohandoss SonaimuthuHui-Fen Wu
May 27, 2021·Biosensors & Bioelectronics·Jun Jie OngAbdul W Basit
Jun 25, 2021·Advanced Healthcare Materials·Kaiyi ZhangXiaodong Chen
Jul 7, 2021·Analytical Methods : Advancing Methods and Applications·Jiaxin ZhangLongshan Zhao
Aug 14, 2021·Analytical Biochemistry·Faezeh TaghaviSeyed Mohammad Taghdisi

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