Design and analysis of a robust genetic Muller C-element

Journal of Theoretical Biology
Nam-phuong NguyenJames Keener

Abstract

This paper presents results on the design and analysis of a robust genetic Muller C-element. The Muller C-element is a standard logic gate commonly used to synchronize independent processes in most asynchronous electronic circuits. Synthetic biological logic gates have been previously demonstrated, but there remain many open issues in the design of sequential (state-holding) logic operations. Three designs are considered for the genetic Muller C-element: a majority gate, a toggle switch, and a speed-independent implementation. While the three designs are logically equivalent, each design requires different assumptions to operate correctly. The majority gate design requires the most timing assumptions, the speed-independent design requires the least, and the toggle switch design is a compromise between the two. This paper examines the robustness of these designs as well as the effects of parameter variation using stochastic simulation. The results show that robustness to timing assumptions does not necessarily increase reliability, suggesting that modifications to existing logic design tools are going to be necessary for synthetic biology. Parameter variation simulations yield further insights into the design principles necessar...Continue Reading

References

Feb 5, 2000·Nature·M B Elowitz, S Leibler
Feb 5, 2000·Nature·T S GardnerJ J Collins
Nov 15, 2002·Nature·Jeff HastyJ J Collins
Oct 7, 2003·Annual Review of Biomedical Engineering·Mads KaernJ J Collins
Nov 25, 2005·Nature·David Sprinzak, Michael B Elowitz
Dec 7, 2005·Journal of Molecular Biology·J Christopher AndersonChristopher A Voigt
Jun 2, 2006·Molecular Systems Biology·Ernesto AndrianantoandroRon Weiss
Jul 25, 2009·Bioinformatics·Chris J MyersNam-Phuong D Nguyen

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Citations

Oct 26, 2011·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Yong ZhangGarng M Huang
Dec 17, 2014·Frontiers in Bioengineering and Biotechnology·Leandro H Watanabe, Chris J Myers
Mar 27, 2018·Natural Computing·Luca CardelliMax Whitby
May 10, 2013·ACS Synthetic Biology·Jason T Stevens, Chris J Myers

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