PMID: 8457432Jan 1, 1993Paper

Design and application of NMR-compatible bioreactor circuits for extended perfusion of high-density mammalian cell cultures

NMR in Biomedicine
R J GilliesB E Dale

Abstract

MR spectroscopy of cultured cells allows non-invasive analyses of the metabolism of cells with specific phenotypes under defined conditions. This technique can be used to investigate the intracellular metabolism of cells or extended to critically evaluate phenomena observed by in vivo MRS. In this paper, a cell maintenance system is described which allows MR analyses with unparalleled spectral resolution, S/N and stability. This system consists of a 25 mm diameter hollow fiber bioreactor and a supporting circuit. The hollow fiber reactor was chosen because it yields a large filling factor which can be perfused through defined volumes. The fibers were 300 microns diameter microporous (0.2 micron) cellulose acetate/cellulose nitrate membranes with high porosity, which allow bulk convective flow throughout the extracapillary space. This flow (Starling flow) is necessary to disrupt steady-state gradients in substrates and waste products. In many respects, the design of the supporting circuit is more important than the bioreactor itself, since it provides the reactor with the proper chemical and physical environment. Hence, this circuit can be applied to a variety of bioreactor configurations. The circuit consists of a hollow fiber ...Continue Reading

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Citations

Jun 3, 1998·NMR in Biomedicine·J M MacdonaldT L James
Feb 7, 2001·The Journal of Gene Medicine·D Pan, C B Whitley
Sep 1, 1994·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·R J GilliesB D Ross
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Apr 9, 2021·Journal of Biochemistry·Kazuhiko YamasakiHiroto Suematsu

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