Design and applications of a self-aligning liquid junction-electrospray interface for capillary electrophoresis-mass spectrometry

Journal of Chromatography. B, Biomedical Applications
T WachsJ D Henion

Abstract

A simple self-aligning liquid junction-electrospray interface for coupling a capillary electrophoresis (CE) system to an atmospheric pressure ionization (API) mass spectrometer (CE-MS) was developed. In contrast to previous liquid junction interfaces, the self-aligning liquid junction interface simplifies the precise alignment of the CE capillary and the sprayer needle and uses a positive make-up flow. Several capillary CE-MS applications were run using both the self-aligning liquid junction interface and the widely used sheath flow interface for comparison purposes. The electrospray stability of the self-aligning liquid junction interface is consistently better even when non-volatile electrolyte solutions are used. At first, some band broadening was obtained with the self-aligning liquid junction interface. Experiments with different CE buffer systems suggested that this band broadening was caused by the materials used in constructing the interface. By using a more inert material for the sprayer needle, the self-aligning liquid junction exhibits excellent electrophoretic resolution, comparable sensitivity, and higher signal-to-noise ratios when run under the same conditions as the sheath flow interface.

Citations

Dec 31, 1998·Electrophoresis·S J KokU A Brinkman
Dec 14, 2001·Journal of Neuroscience Methods·Jonas BergquistJerzy Silberring
May 23, 2012·Journal of Proteomics·Rawi RamautarOleg A Mayboroda
Jun 15, 2007·Journal of Chromatography. a·Rob HaselbergGovert W Somsen
Nov 14, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jieyu ZhouYuxiao Wang
Nov 1, 2008·Mass Spectrometry Reviews·Yehia Mechref, Milos V Novotny
Apr 17, 2012·Journal of the Science of Food and Agriculture·Bahram Mokhtari, Kobra Pourabdollah
Mar 3, 2010·Electrophoresis·E Jane MaxwellDavid D Y Chen
Aug 14, 2012·Proteomics·Jana Krenkova, Frantisek Foret
Mar 9, 2010·Electrophoresis·Zeineb AturkiSalvatore Fanali
Dec 14, 2004·Rapid Communications in Mass Spectrometry : RCM·Charles C LiuNorman J Dovichi
Aug 1, 1997·Analytical Chemistry·R L Sheppard, J Henion
Jul 30, 2014·Electrophoresis·Michael BiacchiEmmanuelle Leize-Wagner

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