Design and chemical synthesis of eukaryotic chromosomes.

Chemical Society Reviews
Ze-Xiong XieYing-Jin Yuan

Abstract

Following the discovery of the DNA double helix structure and the advancement of genome sequencing, we have entered a promising stage with regard to genome writing. Recently, a milestone breakthrough was achieved in the chemical synthesis of designer yeast chromosomes. Here, we review the systematic approaches to the de novo synthesis of designer eukaryotic chromosomes, with an emphasis on technologies and methodologies that enable design, building, testing and debugging. The achievement of chemically synthesized genomes with customized genetic features offers an opportunity to rebuild genome organization, remold biological functions and promote life evolution, which will be of great benefit for application in medicine and industrial manufacturing.

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Citations

Apr 3, 2019·Chembiochem : a European Journal of Chemical Biology·Xiaocui GuoDayong Yang
Sep 10, 2019·Drug Discovery Today·Wilson Wen Bin GohGuillaume Thibault
Jun 26, 2021·Chemical Science·Ze-Xiong XieYing-Jin Yuan
Feb 20, 2019·ACS Synthetic Biology·Duo LiuYing-Jin Yuan
Oct 16, 2021·Chemical Society Reviews·Sijie ZhouYing-Jin Yuan

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Methods Mentioned

BETA
PCA
electrophoresis
flow cytometry
Hi-C
fluorescence-activated cell sorting
PCR

Software Mentioned

SCRaMbLE
SCRaMbLEants
PCRTag
BLAST

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