Abstract
Molecular motors of the cell are protein-based, nanoscale machines, which use a variety of strategies to transduce chemical energy into mechanical work in the presence of a large thermal background. The design and construction of artificial molecular motors is one approach to better understand their basic physical principles. Here, we propose the concept of a protein-based, burnt-bridges ratchet, inspired by biological examples. Our concept, the lawnmower, utilizes protease blades to cleave peptide substrates, and uses the asymmetric substrate-product interface arising from productive cleavage to bias subsequent diffusion on the track (lawn). Following experimental screening to select a protease to act as the motor's blades, we chemically couple trypsin to quantum dots and demonstrate activity of the resulting lawnmower construct in solution. Accompanying Brownian dynamics simulations illustrate the importance for processivity of correct protease density on the quantum dot and spacing of substrates on the track. These results lay the groundwork for future tests of the protein-based lawnmower's motor performance characteristics.
References
Sep 17, 2004·Angewandte Chemie·Peng YinJohn H Reif
Oct 2, 2004·Science·Saveez SaffarianGregory Goldberg
Jun 17, 2005·Angewandte Chemie·Jonathan BathAndrew J Turberfield
May 23, 2006·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Saveez SaffarianGregory Goldberg
Sep 28, 2006·Journal of the American Chemical Society·Renjun PeiMilan N Stojanovic
Jul 26, 2008·Nature Nanotechnology·Jonathan Bath, Andrew J Turberfield
Aug 8, 2008·Nature Nanotechnology·Anita Goel, Viola Vogel
Jul 30, 2009·HFSP Journal·Elizabeth H C BromleyHeiner Linke
Apr 7, 2010·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Laleh SamiiNancy R Forde
May 14, 2010·Nature·Kyle LundHao Yan
Dec 25, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·David ZarkowskyMario Roederer
Feb 8, 2011·Nature Nanotechnology·Shelley F J WickhamAndrew J Turberfield
Mar 19, 2011·Chemical Society Reviews·Max von Delius, David A Leigh
Nov 9, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Laleh SamiiNancy R Forde
Nov 9, 2011·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Nathan J KuwadaHeiner Linke
Feb 22, 2012·Nature Nanotechnology·Lu ChenZev Bryant
Oct 13, 2012·Trends in Cell Biology·Brian S GoodmanSamara L Reck-Peterson
Nov 19, 2013·Nature Nanotechnology·Tony D SchindlerZev Bryant
Feb 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Anthony G VecchiarelliKiyoshi Mizuuchi
Aug 5, 2014·Nature Nanotechnology·Muneaki NakamuraZev Bryant
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Shane R NelsonDavid M Warshaw
Sep 19, 2014·Biomacromolecules·Suzana KovacicNancy R Forde
Nov 5, 2014·Nanoscale·Cassandra S NimanHeiner Linke
Citations
Jul 12, 2020·Biophysics Reviews·Heiner LinkePaul M G Curmi
Dec 22, 2020·Soft Matter·Chapin S KorosecEldon Emberly
May 7, 2021·ACS Nano·Alisina BazrafshanKhalid Salaita
Aug 14, 2019·Nano Letters·Aaron T BlanchardKhalid Salaita
Aug 15, 2019·Chemical Reviews·Aidan I Brown, David A Sivak
Sep 12, 2019·Chemical Reviews·Gadiel Saper, Henry Hess